Rattus norvegicus BN/SHR liver and heart left ventricle ribosomal RNA depleted directional RNA sequencing

Emanuel Wyler, Sebastiaan van Heesch, Eleonora Adami, Norbert Hubner, Markus Landthaler

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: The spontaneously hypertensive rat strain is a frequently used disease model. In a previous study, we measured translational efficiency from this strain and BN-Lx animals. Here, we describe long RNA sequencing reads from ribosomal RNA depleted samples from the same animals. This data can be used to investigate splicing-related events.

RESULTS: RNA was extracted from rat liver and heart left ventricle from BN-Lx and SHR/Ola rats in biological replicates. Ribosomal RNA was removed and the samples subjected to directional high-throughput RNA-sequencing. Read and alignment statistics indicate high quality of the data. The raw sequencing reads are freely available on the NCBI short read archive and can be used for further research on tissue and strain differences, or analysed together with other published high-throughput data from the same animals.

Original languageEnglish
Article number395
Pages (from-to)395
Number of pages1
JournalBMC research notes
Volume10
Issue number1
DOIs
Publication statusPublished - 11 Aug 2017
Externally publishedYes

Keywords

  • Animals
  • Heart Ventricles/metabolism
  • Liver/metabolism
  • Myocardium/metabolism
  • RNA, Messenger/genetics
  • RNA, Ribosomal/genetics
  • Rats
  • Rats, Inbred BN
  • Rats, Inbred SHR
  • Sequence Analysis, RNA/methods
  • Species Specificity

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