TY - JOUR
T1 - Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain
AU - Zelco, Aura
AU - Börjesson, Vanja
AU - De Kanter, Jurrian K.
AU - Lebrero-Fernandez, Cristina
AU - Lauschke, Volker M.
AU - Rocha-Ferreira, Eridan
AU - Nilsson, Gisela
AU - Nair, Syam
AU - Svedin, Pernilla
AU - Bemark, Mats
AU - Hagberg, Henrik
AU - Mallard, Carina
AU - Holstege, Frank C.P.
AU - Wang, Xiaoyang
N1 - Publisher Copyright:
© 2021 Cold Spring Harbor Laboratory Press. All rights reserved.
PY - 2021/8/1
Y1 - 2021/8/1
N2 - The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. Weidentified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.
AB - The meninges are important for brain development and pathology. Using single-cell RNA sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. Weidentified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared with their adult counterparts. We also identified novel meningeal microglia-like cell populations that may participate in white matter development. Early after the hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell resolution view of the importance of meningeal leukocytes at the early stage of development in health and disease.
KW - Meningeal leukocytes
KW - Neonatal mouse
KW - Preterm brain injury
KW - Single-cell RNA sequencing
UR - http://www.scopus.com/inward/record.url?scp=85111934220&partnerID=8YFLogxK
U2 - 10.1101/gad.348190.120
DO - 10.1101/gad.348190.120
M3 - Article
C2 - 34301765
AN - SCOPUS:85111934220
SN - 0890-9369
VL - 35
SP - 1190
EP - 1207
JO - Genes and Development
JF - Genes and Development
IS - 15-16
ER -