Single-cell resolution of morphological changes in hemogenic endothelium

Frank L. Bos, John S. Hawkins, Ann C. Zovein

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Endothelial-to-hematopoietic transition (EHT) occurs within a population of hemogenic endothelial cells during embryogenesis, and leads to the formation of the adult hematopoietic system. Currently, the prospective identification of specific endothelial cells that will undergo EHT, and the cellular events enabling this transition, are not known. We set out to define precisely the morphological events of EHT, and to correlate cellular morphology with the expression of the transcription factors RUNX1 and SOX17. A novel strategy was developed to allow for correlation of immunofluorescence data with the ultrastructural resolution of scanning electron microscopy. The approach can identify single endothelial cells undergoing EHT, as identified by the ratio of RUNX1 to SOX17 immunofluorescence levels, and the morphological changes associated with the transition. Furthermore, this work details a new technical resource that is widely applicable for correlative analyses of single cells in their native tissue environments.

Original languageEnglish
Pages (from-to)2719-2724
Number of pages6
JournalDevelopment
Volume142
Issue number15
DOIs
Publication statusPublished - 1 Aug 2015
Externally publishedYes

Keywords

  • Endothelial-to-hematopoietic transition
  • Endothelium
  • Hematopoiesis
  • Hemogenic
  • Human
  • Imaging
  • Mice
  • RUNX1
  • Scanning electron microscopy
  • SOX17

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