Single cell transcriptomic analysis of the immune cell compartment in the human small intestine and in Celiac disease

Nader Atlasy, Anna Bujko, Espen S. Bækkevold, Peter Brazda, Eva Janssen-Megens, Knut E.A. Lundin, Jørgen Jahnsen, Frode L. Jahnsen, Hendrik G. Stunnenberg

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Celiac disease is an autoimmune disorder in which ingestion of dietary gluten triggers an immune reaction in the small intestine leading to destruction of the lining epithelium. Current treatment focusses on lifelong adherence to a gluten-free diet. Gluten-specific CD4+ T cells and cytotoxic intraepithelial CD8+ T cells have been proposed to be central in disease pathogenesis. Here we use unbiased single-cell RNA-sequencing and explore the heterogeneity of CD45+ immune cells in the human small intestine. We show altered myeloid cell transcriptomes present in active celiac lesions. CD4+ and CD8+ T cells transcriptomes show extensive changes and we define a natural intraepithelial lymphocyte population that is reduced in celiac disease. We show that the immune landscape in Celiac patients on a gluten-free diet is only partially restored compared to control samples. Altogether, we provide a single cell transcriptomic resource that can inform the immune landscape of the small intestine during Celiac disease.

Original languageEnglish
Article number4920
Pages (from-to)4920
JournalNature Communications
Volume13
Issue number1
DOIs
Publication statusPublished - 22 Aug 2022

Keywords

  • CD8-Positive T-Lymphocytes
  • Celiac Disease
  • Glutens
  • Humans
  • Intestine, Small
  • Transcriptome

Fingerprint

Dive into the research topics of 'Single cell transcriptomic analysis of the immune cell compartment in the human small intestine and in Celiac disease'. Together they form a unique fingerprint.

Cite this