Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

Anne E. Bras; Zgjim Osmani; Valerie de Haas; Mojca Jongen-Lavrencic; Jeroen G. te Marvelde; C. Michel Zwaan; Ester Mejstrikova; Paula Fernandez; Tomasz Szczepanski; Alberto Orfao; Jacques J.M. van Dongen; Vincent H.J. van der Velden

doi:10.1111/bjh.17210

Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

Anne E. Bras
, Zgjim Osmani
, Valerie de Haas
, Mojca Jongen-Lavrencic
, Jeroen G. te Marvelde
, C. Michel Zwaan
, Ester Mejstrikova
, Paula Fernandez
, Tomasz Szczepanski
, Alberto Orfao
, Jacques J.M. van Dongen
, Vincent H.J. van der Velden

Group Zwaan

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.

Original language	English
Pages (from-to)	922-927
Number of pages	6
Journal	British Journal of Haematology
Volume	193
Issue number	5
DOIs	https://doi.org/10.1111/bjh.17210
Publication status	Published - Jun 2021

Keywords

acute leukaemia
ALL
AML
flow cytometry
immunophenotyping
myeloperoxidase

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10.1111/bjh.17210

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Bras, A. E., Osmani, Z., de Haas, V., Jongen-Lavrencic, M., te Marvelde, J. G., Zwaan, C. M., Mejstrikova, E., Fernandez, P., Szczepanski, T., Orfao, A., van Dongen, J. J. M., & van der Velden, V. H. J. (2021). Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia. British Journal of Haematology, 193(5), 922-927. https://doi.org/10.1111/bjh.17210

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title = "Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia",

abstract = "Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20\% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.",

keywords = "acute leukaemia, ALL, AML, flow cytometry, immunophenotyping, myeloperoxidase",

author = "Bras, \{Anne E.\} and Zgjim Osmani and \{de Haas\}, Valerie and Mojca Jongen-Lavrencic and \{te Marvelde\}, \{Jeroen G.\} and Zwaan, \{C. Michel\} and Ester Mejstrikova and Paula Fernandez and Tomasz Szczepanski and Alberto Orfao and \{van Dongen\}, \{Jacques J.M.\} and \{van der Velden\}, \{Vincent H.J.\}",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors. British Journal of Haematology published by British Society for Haematology and John Wiley \& Sons Ltd.",

year = "2021",

month = jun,

doi = "10.1111/bjh.17210",

language = "English",

volume = "193",

pages = "922--927",

journal = "British Journal of Haematology",

issn = "0007-1048",

publisher = "John Wiley and Sons Inc.",

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T1 - Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

AU - Bras, Anne E.

AU - Osmani, Zgjim

AU - de Haas, Valerie

AU - Jongen-Lavrencic, Mojca

AU - te Marvelde, Jeroen G.

AU - Zwaan, C. Michel

AU - Mejstrikova, Ester

AU - Fernandez, Paula

AU - Szczepanski, Tomasz

AU - Orfao, Alberto

AU - van Dongen, Jacques J.M.

AU - van der Velden, Vincent H.J.

PY - 2021/6

Y1 - 2021/6

N2 - Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.

AB - Given its myeloid-restricted expression, myeloperoxidase (MPO) is typically used for lineage assignment (myeloid vs. lymphoid) during acute leukaemia (AL) diagnostics. In the present study, a robust flow cytometric definition for MPO positivity was established based on the standardised EuroFlow protocols, the standardised Acute Leukaemia Orientation Tube and 1734 multicentre AL cases (with confirmed assay stability). The best diagnostic performance was achieved by defining MPO positivity as ≥20% of the AL cells exceeding a lymphocyte-based threshold. The methodology employed should be applicable to any form of standardised flow cytometry.

KW - acute leukaemia

KW - ALL

KW - AML

KW - flow cytometry

KW - immunophenotyping

KW - myeloperoxidase

UR - https://www.scopus.com/pages/publications/85096637278

U2 - 10.1111/bjh.17210

DO - 10.1111/bjh.17210

M3 - Article

C2 - 33161592

AN - SCOPUS:85096637278

SN - 0007-1048

VL - 193

SP - 922

EP - 927

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 5

ER -

Standardised immunophenotypic analysis of myeloperoxidase in acute leukaemia

Abstract

Keywords

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