Structure-function relationship of hammerhead ribozymes as probed by 2'-modifications

W A Pieken, D B Olsen, H Aurup, D M Williams, O Heidenreich, F Benseler, F Eckstein

Research output: Contribution to journalArticlepeer-review

Abstract

Hammerhead ribozymes containing 2'-fluoro- or 2'-aminonucleotides were prepared by automated chemical synthesis. Incorporation of 2'-fluorouridines, 2'-fluorocytidines or 2'-aminouridines did not appreciably decrease catalytic activity. The presence of 2'-aminocytidines, however, reduced the activity about 20-fold. No catalytic activity could be measured for ribozymes in which all adenosines were replaced by the 2'-fluoro analogue in presence of MgCl2. No single position could be found responsible for this loss of activity. In an attempt to construct ribozymes to hydrolyse HIV-RNA in the 5'-LTR region several constructs were tested on synthetic substrate as well as on run-off transcripts of about 1000 nucleotides length.

Original languageEnglish
Pages (from-to)51-3
Number of pages3
JournalNucleic acids symposium series
Issue number24
Publication statusPublished - 1991
Externally publishedYes

Keywords

  • Antiviral Agents/chemical synthesis
  • Base Sequence
  • HIV/drug effects
  • HIV Long Terminal Repeat
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Oligoribonucleotides/chemical synthesis
  • RNA, Catalytic/chemical synthesis
  • RNA, Viral/drug effects
  • Ribonucleotides

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