Abstract
Hammerhead ribozymes containing 2'-fluoro- or 2'-aminonucleotides were prepared by automated chemical synthesis. Incorporation of 2'-fluorouridines, 2'-fluorocytidines or 2'-aminouridines did not appreciably decrease catalytic activity. The presence of 2'-aminocytidines, however, reduced the activity about 20-fold. No catalytic activity could be measured for ribozymes in which all adenosines were replaced by the 2'-fluoro analogue in presence of MgCl2. No single position could be found responsible for this loss of activity. In an attempt to construct ribozymes to hydrolyse HIV-RNA in the 5'-LTR region several constructs were tested on synthetic substrate as well as on run-off transcripts of about 1000 nucleotides length.
Original language | English |
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Pages (from-to) | 51-3 |
Number of pages | 3 |
Journal | Nucleic acids symposium series |
Issue number | 24 |
Publication status | Published - 1991 |
Externally published | Yes |
Keywords
- Antiviral Agents/chemical synthesis
- Base Sequence
- HIV/drug effects
- HIV Long Terminal Repeat
- Molecular Sequence Data
- Nucleic Acid Conformation
- Oligoribonucleotides/chemical synthesis
- RNA, Catalytic/chemical synthesis
- RNA, Viral/drug effects
- Ribonucleotides