TY - JOUR
T1 - TAC, a TBP-sans-TAFs complex containing the unprocessed TFIIAαβ precursor and the TFIIAγ subunit
AU - Mitsiou, Dimitra J.
AU - Stunnenberg, Hendrik G.
N1 - Funding Information:
We thank J. Betz, C. Logie, and members of the Stunnenberg laboratory for continued discussions and critical reading of the manuscript, and P. Doesburg, E. Kitraki, and M. Aerts for assistance. We thank L. Tora, I. Davidson, H. T. Timmers, P. M. Lieberman, J. Ozer, D. Reinberg, R. G. Roeder, R. J. White, I. Grummt, S. Gunderson, and particularly N. Hernandez for kindly providing plasmids and antibodies. D. J. M. was supported by an EU Marie Curie Fellowship. This project was supported by the EU-TMR Network grant ERB-FMRX-CT96-0064.
PY - 2000
Y1 - 2000
N2 - Transcription of TATA box-containing genes by RNA polymerase II is mediated by TBP-containing and TBP-free multisubunit complexes consisting of common and unique components. We have identified a highly stable TBP-TFIIA-containing complex, TAC, which is detectable in embryonal carcinoma (EC) cells but not in differentiated cells. TAC contains the TFIIAγ subunit and the unprocessed form of TFIIAαβ, although the processed TFIIAα and TFIIAβ subunits are present in EC cells. TAC mediates transcriptional activation by RNA polymerase II in vivo, even though it does not contain classical TAFs. Formaldehyde cross-linking revealed that in EC but not in differentiated cells, association of TBP with chromatin is strongly enhanced when complexed with TFIIA in vivo. Remarkably, the TFIIAαβ precursor is preferentially, if not exclusively, associated with chromatin as compared to the processed subunits present in 'free' TFIIA in EC cells.
AB - Transcription of TATA box-containing genes by RNA polymerase II is mediated by TBP-containing and TBP-free multisubunit complexes consisting of common and unique components. We have identified a highly stable TBP-TFIIA-containing complex, TAC, which is detectable in embryonal carcinoma (EC) cells but not in differentiated cells. TAC contains the TFIIAγ subunit and the unprocessed form of TFIIAαβ, although the processed TFIIAα and TFIIAβ subunits are present in EC cells. TAC mediates transcriptional activation by RNA polymerase II in vivo, even though it does not contain classical TAFs. Formaldehyde cross-linking revealed that in EC but not in differentiated cells, association of TBP with chromatin is strongly enhanced when complexed with TFIIA in vivo. Remarkably, the TFIIAαβ precursor is preferentially, if not exclusively, associated with chromatin as compared to the processed subunits present in 'free' TFIIA in EC cells.
UR - https://www.scopus.com/pages/publications/0033638227
U2 - 10.1016/S1097-2765(00)00052-6
DO - 10.1016/S1097-2765(00)00052-6
M3 - Article
C2 - 11030333
AN - SCOPUS:0033638227
SN - 1097-2765
VL - 6
SP - 527
EP - 537
JO - Molecular Cell
JF - Molecular Cell
IS - 3
ER -