The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

Martin Hagenaars; Ron Koelemij; N. Geeske Ensink; Jaap D.H. Van Eendenburg; Ronald L.P. Van Vlierberghe; Alexander M.M. Eggermont; Cornelis J.H. Van de Velde; Gert Jan Fleuren; Peter J.K. Kuppen

doi:10.1023/A:1011062002851

The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

Martin Hagenaars
, Ron Koelemij
, N. Geeske Ensink
, Jaap D.H. Van Eendenburg
, Ronald L.P. Van Vlierberghe
, Alexander M.M. Eggermont
, Cornelis J.H. Van de Velde
, Gert Jan Fleuren
, Peter J.K. Kuppen

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.

Original language	English
Pages (from-to)	281-289
Number of pages	9
Journal	Clinical and Experimental Metastasis
Volume	18
Issue number	4
DOIs	https://doi.org/10.1023/A:1011062002851
Publication status	Published - 2000
Externally published	Yes

Keywords

Colon tumour
Monoclonal antibody
Rat

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Hagenaars, M., Koelemij, R., Ensink, N. G., Van Eendenburg, J. D. H., Van Vlierberghe, R. L. P., Eggermont, A. M. M., Van de Velde, C. J. H., Fleuren, G. J., & Kuppen, P. J. K. (2000). The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma. Clinical and Experimental Metastasis, 18(4), 281-289. https://doi.org/10.1023/A:1011062002851

@article{3044f0e6481145d88c4696c3c3376111,

title = "The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma",

abstract = "In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.",

keywords = "Colon tumour, Monoclonal antibody, Rat",

author = "Martin Hagenaars and Ron Koelemij and Ensink, \{N. Geeske\} and \{Van Eendenburg\}, \{Jaap D.H.\} and \{Van Vlierberghe\}, \{Ronald L.P.\} and Eggermont, \{Alexander M.M.\} and \{Van de Velde\}, \{Cornelis J.H.\} and Fleuren, \{Gert Jan\} and Kuppen, \{Peter J.K.\}",

note = "Funding Information: This work was supported by the Dutch Cancer Society, grant number RUL 94-784. We also thank Mrs H.A.M. Holtslag and Mrs M. van Brakel for excellent technical assistance.",

year = "2000",

doi = "10.1023/A:1011062002851",

language = "English",

volume = "18",

pages = "281--289",

journal = "Clinical and Experimental Metastasis",

issn = "0262-0898",

publisher = "Springer Science and Business Media B.V.",

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T1 - The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

AU - Hagenaars, Martin

AU - Koelemij, Ron

AU - Ensink, N. Geeske

AU - Van Eendenburg, Jaap D.H.

AU - Van Vlierberghe, Ronald L.P.

AU - Eggermont, Alexander M.M.

AU - Van de Velde, Cornelis J.H.

AU - Fleuren, Gert Jan

AU - Kuppen, Peter J.K.

N1 - Funding Information: This work was supported by the Dutch Cancer Society, grant number RUL 94-784. We also thank Mrs H.A.M. Holtslag and Mrs M. van Brakel for excellent technical assistance.

PY - 2000

Y1 - 2000

N2 - In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.

AB - In this paper we describe 4 new monoclonal antibodies to be applied in rat models for cancer. The monoclonal antibodies were obtained by immunizing Balb/c mice with CC531 rat colon adenocarcinoma cells. Hybridomas were produced and 4 were selected for their reactivity with CC531 in vitro (MG1, 2, 3 and 4). All 4 antibodies recognized other rat tumour cell lines and showed limited cross-reactivity with normal rat tissues. Intraperitoneally injected MG1, 2 and 4 homed to in vivo growing, artificially induced CC531 liver metastases. In these in vivo experiments, limited cross-reactivity with normal rat tissues, predominantly of the gastro-intestinal tract, was found. MG4 was found to enhance lysis of CC531 tumour cells mediated by IL-2 activated, cultured natural killer cells. These antibodies are potentially useful for antibody-based laboratory techniques and for investigation of antibody-based immunotherapy of cancer in a rat model.

KW - Colon tumour

KW - Monoclonal antibody

KW - Rat

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U2 - 10.1023/A:1011062002851

DO - 10.1023/A:1011062002851

M3 - Article

C2 - 11448057

AN - SCOPUS:0034961058

SN - 0262-0898

VL - 18

SP - 281

EP - 289

JO - Clinical and Experimental Metastasis

JF - Clinical and Experimental Metastasis

IS - 4

ER -

The development of novel mouse monoclonal antibodies against the CC531 rat colon adenocarcinoma

Abstract

Keywords

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