TY - JOUR
T1 - The DNA damage response
T2 - The omics era and its impact
AU - Derks, Kasper W.J.
AU - Hoeijmakers, Jan H.J.
AU - Pothof, Joris
N1 - Funding Information:
We acknowledge financial support from the European Commission FP7-Health-2008-200880 , HEALTH-F2-2010-259893 and National Institute of Health/National Institute of Ageing ( 1PO1 AG-17242-02 ), NIEHS ( 1UO1 ES011044 ), and the Royal Academy of Arts and Sciences of The Netherlands (ISK/2483/PAH) (academia professorship to JHJH), European Research Council (233424) Advanced Grant to JHJH, a KWO grant from the Dutch Cancer Society (2011-5030) and The Netherlands Toxicogenomics Center (050-040-203).
PY - 2014/7
Y1 - 2014/7
N2 - The emergence of high density technologies monitoring the genome, transcriptome and proteome in relation to genotoxic stress have tremendously enhanced our knowledge on global responses and dynamics in the DNA damage response, including its relation with cancer and aging. Moreover, '-omics' technologies identified many novel factors, their post-translational modifications, pathways and global responses in the cellular response to DNA damage. Based on omics, it is currently estimated that thousands of gene(product)s participate in the DNA damage response, recognizing complex networks that determine cell fate after damage to the most precious cellular molecule, DNA. The development of next generation sequencing technology and associated specialized protocols can quantitatively monitor RNA and DNA at unprecedented single nucleotide resolution. In this review we will discuss the contribution of omics technologies and in particular next generation sequencing to our understanding of the DNA damage response and the future prospective of next generation sequencing, its single cell application and omics dataset integration in unraveling intricate DNA damage signaling networks.
AB - The emergence of high density technologies monitoring the genome, transcriptome and proteome in relation to genotoxic stress have tremendously enhanced our knowledge on global responses and dynamics in the DNA damage response, including its relation with cancer and aging. Moreover, '-omics' technologies identified many novel factors, their post-translational modifications, pathways and global responses in the cellular response to DNA damage. Based on omics, it is currently estimated that thousands of gene(product)s participate in the DNA damage response, recognizing complex networks that determine cell fate after damage to the most precious cellular molecule, DNA. The development of next generation sequencing technology and associated specialized protocols can quantitatively monitor RNA and DNA at unprecedented single nucleotide resolution. In this review we will discuss the contribution of omics technologies and in particular next generation sequencing to our understanding of the DNA damage response and the future prospective of next generation sequencing, its single cell application and omics dataset integration in unraveling intricate DNA damage signaling networks.
KW - DNA damage response
KW - Genomics
KW - Next generation sequencing
KW - Proteomics
KW - Transcriptomics
UR - https://www.scopus.com/pages/publications/84902081860
U2 - 10.1016/j.dnarep.2014.03.008
DO - 10.1016/j.dnarep.2014.03.008
M3 - Article
C2 - 24794401
AN - SCOPUS:84902081860
SN - 1568-7864
VL - 19
SP - 214
EP - 220
JO - DNA repair
JF - DNA repair
ER -