Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13

R. J. Sinke; M. Van Asseldonk; D. De Bruijn; J. A. Strijk; G. Merkx; D. Olde Weghuis; B. De Jong; J. W. Oosterhuis; A. Geurts Van Kessel

doi:10.1111/j.1699-0463.1998.tb01321.x

Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13

R. J. Sinke, M. Van Asseldonk, D. De Bruijn, J. A. Strijk, G. Merkx, D. Olde Weghuis, B. De Jong, J. W. Oosterhuis, A. Geurts Van Kessel

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4 Citations (Scopus)

Abstract

In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.

Original language	English
Pages (from-to)	73-79
Number of pages	7
Journal	APMIS
Volume	106
Issue number	1
DOIs	https://doi.org/10.1111/j.1699-0463.1998.tb01321.x
Publication status	Published - 1998
Externally published	Yes

Keywords

Chromosome 11q13 breakpoints
Extragonadal germ cell tumours

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10.1111/j.1699-0463.1998.tb01321.x

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abstract = "In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.",

keywords = "Chromosome 11q13 breakpoints, Extragonadal germ cell tumours",

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doi = "10.1111/j.1699-0463.1998.tb01321.x",

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T1 - Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13

AU - Sinke, R. J.

AU - Van Asseldonk, M.

AU - De Bruijn, D.

AU - Strijk, J. A.

AU - Merkx, G.

AU - Olde Weghuis, D.

AU - De Jong, B.

AU - Oosterhuis, J. W.

AU - Geurts Van Kessel, A.

PY - 1998

Y1 - 1998

N2 - In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.

AB - In a previous study we have defined a subgroup of human malignant extragonadal germ cell tumours that is characterized by complex translocations involving chromosomes 6 and 11 (Echten et al. 1995). Here we report (i) the use of fluorescent in situ hybridization, pulsed field gel electrophoresis and direct visual hybridization techniques to localize the tumour-associated breakpoint within band 11q13, and (ii) the construction of a phage library enriched for this region to facilitate genomic walks towards the breakpoint. Extensive breakpoint-flanking contigs were generated and within these contigs six candidate genes could be identified.

KW - Chromosome 11q13 breakpoints

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Towards the isolation of a human malignant extragonadal germ cell tumour-associated breakpoint in chromosome 11q13

Abstract

Keywords

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