Transforming growth factor beta secretion from primary breast cancer fibroblasts

C. E.P. van Roozendaal; J. G.M. Klijn; B. van Ooijen; C. Claassen; A. M.M. Eggermont; S. C. Henzen-Logmans; J. A. Foekens

doi:10.1016/0303-7207(95)03539-J

Transforming growth factor beta secretion from primary breast cancer fibroblasts

C. E.P. van Roozendaal, J. G.M. Klijn, B. van Ooijen, C. Claassen, A. M.M. Eggermont, S. C. Henzen-Logmans, J. A. Foekens

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β₁ has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β₁ were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β₁ secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Molecular and Cellular Endocrinology
Volume	111
Issue number	1
DOIs	https://doi.org/10.1016/0303-7207(95)03539-J
Publication status	Published - 28 Apr 1995
Externally published	Yes

Keywords

Fibroblast
Human breast cells
Tamoxifen
Transforming growth factor-beta

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10.1016/0303-7207(95)03539-J

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title = "Transforming growth factor beta secretion from primary breast cancer fibroblasts",

abstract = "Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.",

keywords = "Fibroblast, Human breast cells, Tamoxifen, Transforming growth factor-beta",

author = "\{van Roozendaal\}, \{C. E.P.\} and Klijn, \{J. G.M.\} and \{van Ooijen\}, B. and C. Claassen and Eggermont, \{A. M.M.\} and Henzen-Logmans, \{S. C.\} and Foekens, \{J. A.\}",

note = "Funding Information: We gratefully acknowledge Maxime P. Look and Wim L.J. van Putten for statistical analysis. This study was supported by the Dutch Cancer Society (grant DDHK 91-15).",

year = "1995",

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doi = "10.1016/0303-7207(95)03539-J",

language = "English",

volume = "111",

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T1 - Transforming growth factor beta secretion from primary breast cancer fibroblasts

AU - van Roozendaal, C. E.P.

AU - Klijn, J. G.M.

AU - van Ooijen, B.

AU - Claassen, C.

AU - Eggermont, A. M.M.

AU - Henzen-Logmans, S. C.

AU - Foekens, J. A.

N1 - Funding Information: We gratefully acknowledge Maxime P. Look and Wim L.J. van Putten for statistical analysis. This study was supported by the Dutch Cancer Society (grant DDHK 91-15).

PY - 1995/4/28

Y1 - 1995/4/28

N2 - Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

AB - Transforming growth factor beta (TGF-β) is a hormonally regulated growth inhibitor with autocrine and/or paracrine functions in human breast cancer. In vivo, enhanced immunohistochemical staining of extracellular TGF-β1 has been detected around stromal fibroblasts in response to the antiestrogen treatment. We have investigated the effects of tamoxifen on the production of TGF-β by primary human breast fibroblast cultures in serum-free medium. Highly variable levels of mainly latent TGF-β1 were detected in conditioned media from both tumor and normal tissue derived fibroblasts. Hydroxy-tamoxifen was shown to increase latent TGF-β1 secretion in three of the eight tumor tissue-derived fibroblast cultures. Such effect of hydroxy-tamoxifen was not observed in fibroblast cultures established from normal adjacent breast tissue.

KW - Fibroblast

KW - Human breast cells

KW - Tamoxifen

KW - Transforming growth factor-beta

UR - https://www.scopus.com/pages/publications/0029033765

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DO - 10.1016/0303-7207(95)03539-J

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AN - SCOPUS:0029033765

SN - 0303-7207

VL - 111

SP - 1

EP - 6

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 1

ER -

Transforming growth factor beta secretion from primary breast cancer fibroblasts

Abstract

Keywords

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