Transmaternal cell flow leads to antigen-experienced cord blood

Miranda P. Dierselhuis; Els C. Blokland; Jos Pool; Ellen Schrama; Sicco A. Scherjon; Els Goulmy

doi:10.1182/blood-2012-02-410571

Transmaternal cell flow leads to antigen-experienced cord blood

Miranda P. Dierselhuis, Els C. Blokland, Jos Pool, Ellen Schrama, Sicco A. Scherjon, Els Goulmy

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Umbilical cord blood (UCB) is used for HSCT. It is known that UCB can comprise Ag-specific T cells. Here we question whether solely transmaternal cell flow may immunize UCB. Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. Forty-two of 104 tetramerpos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. Male microchimerism was present in 6 of 12 UCB samples analyzed. In conclusion, female UCB comprises HY-specific cytotoxic T cells. The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors.

Original language	English
Pages (from-to)	505-510
Number of pages	6
Journal	Blood
Volume	120
Issue number	3
DOIs	https://doi.org/10.1182/blood-2012-02-410571
Publication status	Published - 19 Jul 2012
Externally published	Yes

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title = "Transmaternal cell flow leads to antigen-experienced cord blood",

abstract = "Umbilical cord blood (UCB) is used for HSCT. It is known that UCB can comprise Ag-specific T cells. Here we question whether solely transmaternal cell flow may immunize UCB. Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. Forty-two of 104 tetramerpos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. Male microchimerism was present in 6 of 12 UCB samples analyzed. In conclusion, female UCB comprises HY-specific cytotoxic T cells. The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors.",

author = "Dierselhuis, {Miranda P.} and Blokland, {Els C.} and Jos Pool and Ellen Schrama and Scherjon, {Sicco A.} and Els Goulmy",

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doi = "10.1182/blood-2012-02-410571",

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T1 - Transmaternal cell flow leads to antigen-experienced cord blood

AU - Dierselhuis, Miranda P.

AU - Blokland, Els C.

AU - Pool, Jos

AU - Schrama, Ellen

AU - Scherjon, Sicco A.

AU - Goulmy, Els

PY - 2012/7/19

Y1 - 2012/7/19

N2 - Umbilical cord blood (UCB) is used for HSCT. It is known that UCB can comprise Ag-specific T cells. Here we question whether solely transmaternal cell flow may immunize UCB. Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. Forty-two of 104 tetramerpos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. Male microchimerism was present in 6 of 12 UCB samples analyzed. In conclusion, female UCB comprises HY-specific cytotoxic T cells. The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors.

AB - Umbilical cord blood (UCB) is used for HSCT. It is known that UCB can comprise Ag-specific T cells. Here we question whether solely transmaternal cell flow may immunize UCB. Twenty-three female UCB samples were collected from healthy mothers and analyzed for minor histocompatibility Ag HY-specific responses. Forty-two of 104 tetramerpos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. Male microchimerism was present in 6 of 12 UCB samples analyzed. In conclusion, female UCB comprises HY-specific cytotoxic T cells. The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors.

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Transmaternal cell flow leads to antigen-experienced cord blood

Abstract

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