Use of in vivo and in vitro assays for the characterization of mammalian excision repair and isolation of repair proteins

J. H.J. Hoeijmakers; A. P.M. Eker; R. D. Wood; P. Robins

doi:10.1016/0921-8777(90)90007-R

Use of in vivo and in vitro assays for the characterization of mammalian excision repair and isolation of repair proteins

J. H.J. Hoeijmakers, A. P.M. Eker, R. D. Wood, P. Robins

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

Elucidation of the molecular mechanism of mammalian nucleotide excision repair requires the availability of purified proteins, DNA substrates with defined lesions and suitable repair assays. Repair assays introduced in recent years vary from testing individual steps and successions of steps in vitro to systems that closely reflect the entire process in vivo. In the first part of this review, an in vivo microinjection system is discussed. The second part of the article reviews an in vitro system for study of repair synthesis promoted by cell extracts. Both systems can be utilized as assays during the purification of protein factors that complement repair-defective xeroderma pigmentosum cells. The effect of purified repair proteins from other organisms on mammalian repair is also considered.

Original language	English
Pages (from-to)	223-238
Number of pages	16
Journal	Mutation Research-DNA Repair
Volume	236
Issue number	2-3
DOIs	https://doi.org/10.1016/0921-8777(90)90007-R
Publication status	Published - 1990
Externally published	Yes

Keywords

DNA repair
Nucleotide excision repair
Proteins, in DNA repair
Xeroderma pigmentosum

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10.1016/0921-8777(90)90007-R

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title = "Use of in vivo and in vitro assays for the characterization of mammalian excision repair and isolation of repair proteins",

abstract = "Elucidation of the molecular mechanism of mammalian nucleotide excision repair requires the availability of purified proteins, DNA substrates with defined lesions and suitable repair assays. Repair assays introduced in recent years vary from testing individual steps and successions of steps in vitro to systems that closely reflect the entire process in vivo. In the first part of this review, an in vivo microinjection system is discussed. The second part of the article reviews an in vitro system for study of repair synthesis promoted by cell extracts. Both systems can be utilized as assays during the purification of protein factors that complement repair-defective xeroderma pigmentosum cells. The effect of purified repair proteins from other organisms on mammalian repair is also considered.",

keywords = "DNA repair, Nucleotide excision repair, Proteins, in DNA repair, Xeroderma pigmentosum",

author = "Hoeijmakers, \{J. H.J.\} and Eker, \{A. P.M.\} and Wood, \{R. D.\} and P. Robins",

note = "Funding Information: The work of J.H. was in part supported by the Commission of the European Community contract Funding Information: no. B16-141-NL, and Medigon, Foundation of Medical and Health Research in The Netherlands contract no. 900-501-091. A.E. was supported by the Technical University Delft. A.E. and J.H. are very indebted to Mr, W. Vermeulen for invaluable help with the (tedious) microneedle injection experiments, Dr. L. Roza for the immunofluorescence data, Dr. N.G.J. Jaspers for useful suggestions, Prof. D. Bootsma for continuous support, and Rita Boucke for rapid and skilful typing of part of the manuscript. R.W. and P.R. were supported by the Imperial Cancer Research Fund. R.W. and P.R. thank Dr. T. Lindahl for his valuable continuing collaboration and support in the study of the biochemistry of XP, and thank our colleagues Dr. J. Hansson, Ms. D. Coverley, Ms. M. Biggerstaff, Dr. G. Frosina and Dr. S. Keyse for many valuable interactions.",

year = "1990",

doi = "10.1016/0921-8777(90)90007-R",

language = "English",

volume = "236",

pages = "223--238",

journal = "Mutation Research-DNA Repair",

issn = "0921-8777",

publisher = "Elsevier B.V.",

number = "2-3",

}

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AU - Hoeijmakers, J. H.J.

AU - Eker, A. P.M.

AU - Wood, R. D.

AU - Robins, P.

N1 - Funding Information: The work of J.H. was in part supported by the Commission of the European Community contract Funding Information: no. B16-141-NL, and Medigon, Foundation of Medical and Health Research in The Netherlands contract no. 900-501-091. A.E. was supported by the Technical University Delft. A.E. and J.H. are very indebted to Mr, W. Vermeulen for invaluable help with the (tedious) microneedle injection experiments, Dr. L. Roza for the immunofluorescence data, Dr. N.G.J. Jaspers for useful suggestions, Prof. D. Bootsma for continuous support, and Rita Boucke for rapid and skilful typing of part of the manuscript. R.W. and P.R. were supported by the Imperial Cancer Research Fund. R.W. and P.R. thank Dr. T. Lindahl for his valuable continuing collaboration and support in the study of the biochemistry of XP, and thank our colleagues Dr. J. Hansson, Ms. D. Coverley, Ms. M. Biggerstaff, Dr. G. Frosina and Dr. S. Keyse for many valuable interactions.

PY - 1990

Y1 - 1990

N2 - Elucidation of the molecular mechanism of mammalian nucleotide excision repair requires the availability of purified proteins, DNA substrates with defined lesions and suitable repair assays. Repair assays introduced in recent years vary from testing individual steps and successions of steps in vitro to systems that closely reflect the entire process in vivo. In the first part of this review, an in vivo microinjection system is discussed. The second part of the article reviews an in vitro system for study of repair synthesis promoted by cell extracts. Both systems can be utilized as assays during the purification of protein factors that complement repair-defective xeroderma pigmentosum cells. The effect of purified repair proteins from other organisms on mammalian repair is also considered.

AB - Elucidation of the molecular mechanism of mammalian nucleotide excision repair requires the availability of purified proteins, DNA substrates with defined lesions and suitable repair assays. Repair assays introduced in recent years vary from testing individual steps and successions of steps in vitro to systems that closely reflect the entire process in vivo. In the first part of this review, an in vivo microinjection system is discussed. The second part of the article reviews an in vitro system for study of repair synthesis promoted by cell extracts. Both systems can be utilized as assays during the purification of protein factors that complement repair-defective xeroderma pigmentosum cells. The effect of purified repair proteins from other organisms on mammalian repair is also considered.

KW - DNA repair

KW - Nucleotide excision repair

KW - Proteins, in DNA repair

KW - Xeroderma pigmentosum

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AN - SCOPUS:0025120573

SN - 0921-8777

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JO - Mutation Research-DNA Repair

JF - Mutation Research-DNA Repair

IS - 2-3

ER -

Use of in vivo and in vitro assays for the characterization of mammalian excision repair and isolation of repair proteins

Abstract

Keywords

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