Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains

P. Borst; F. Fase-Fowler; J. H.J. Hoeijmakers; A. C.C. Frasch

doi:10.1016/0005-2787(80)90001-5

Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains

P. Borst
, F. Fase-Fowler
, J. H.J. Hoeijmakers
, A. C.C. Frasch

Research output: Contribution to journal › Article › peer-review

55 Citations (Scopus)

Abstract

We have compared a total of 30 recognition sites for eight restriction endonucleases on the 20-kilobase-pair maxi-circle of kinetoplast DNAs from five different Trypanosoma brucei strains. In addition to three polymorphic sites we have found a 5 kilobase-pair region that is not cleaved by any of the eight enzymes and that varies in size over 1 kilobase pair in the strains analysed. Mini-circles from these five strains, digested with endonuclease TaqI or MboII, yield very complex fragment patterns, showing that extensive mini-circle sequence heterogeneity is a common characteristic of these T. brucei strains. The size distribution of mini-circle fragments in these digests was identical for different clones of the 427 strain, but very different for mini-circles from different strains. These results show that maxi-circle sequence is conserved, whereas mini-circle sequence is not. Restriction digests of maxi-circles could be useful in determining how closely two Trypanosoma strains are related, whereas mini-circle digests can serve as sensitive tags for individual strains.

Original language	English
Pages (from-to)	197-210
Number of pages	14
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	610
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(80)90001-5
Publication status	Published - 11 Dec 1980
Externally published	Yes

Keywords

(Trypanosoma brucei)
Maxi-circle
Mini-circle
Nucleotide sequence
Restriction endonuclease
kinetoplast DNA

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10.1016/0005-2787(80)90001-5

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@article{f73fd4f646884996b76a66c92a0be050,

title = "Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains",

abstract = "We have compared a total of 30 recognition sites for eight restriction endonucleases on the 20-kilobase-pair maxi-circle of kinetoplast DNAs from five different Trypanosoma brucei strains. In addition to three polymorphic sites we have found a 5 kilobase-pair region that is not cleaved by any of the eight enzymes and that varies in size over 1 kilobase pair in the strains analysed. Mini-circles from these five strains, digested with endonuclease TaqI or MboII, yield very complex fragment patterns, showing that extensive mini-circle sequence heterogeneity is a common characteristic of these T. brucei strains. The size distribution of mini-circle fragments in these digests was identical for different clones of the 427 strain, but very different for mini-circles from different strains. These results show that maxi-circle sequence is conserved, whereas mini-circle sequence is not. Restriction digests of maxi-circles could be useful in determining how closely two Trypanosoma strains are related, whereas mini-circle digests can serve as sensitive tags for individual strains.",

keywords = "(Trypanosoma brucei), Maxi-circle, Mini-circle, Nucleotide sequence, Restriction endonuclease, kinetoplast DNA",

author = "P. Borst and F. Fase-Fowler and Hoeijmakers, \{J. H.J.\} and Frasch, \{A. C.C.\}",

note = "Funding Information: We are indebted to several colleagues, specified in Materials and Methods, for T. brucei strains; to Dr. J. Davison, Research Unit of Molecular Biology, International Institute of Cellular and Molecular Pathology, Brussels, Belgium, for providing cloned DNA, to Miss A. Snijders for carrying out the hybridization experiment of Fig. 3 and to Miss J. van den Burg for help in some experiments. This work was supported in part by a grant to P.B. from the Foundation for Fundamental Biological Research (BION), which is subsidized by The Netherlands Organization for the Advancement of Pure Research (ZWO). Initial experiments were supported by a grant to P.B. from NATO (grant Nr-559). A.C.C.F. gratefully acknowledges a research training grant from the World Health Organization (WHO No. M8/181/4/F.78).",

year = "1980",

month = dec,

day = "11",

doi = "10.1016/0005-2787(80)90001-5",

language = "English",

volume = "610",

pages = "197--210",

journal = "BBA Section Nucleic Acids And Protein Synthesis",

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number = "2",

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TY - JOUR

T1 - Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains

AU - Borst, P.

AU - Fase-Fowler, F.

AU - Hoeijmakers, J. H.J.

AU - Frasch, A. C.C.

N1 - Funding Information: We are indebted to several colleagues, specified in Materials and Methods, for T. brucei strains; to Dr. J. Davison, Research Unit of Molecular Biology, International Institute of Cellular and Molecular Pathology, Brussels, Belgium, for providing cloned DNA, to Miss A. Snijders for carrying out the hybridization experiment of Fig. 3 and to Miss J. van den Burg for help in some experiments. This work was supported in part by a grant to P.B. from the Foundation for Fundamental Biological Research (BION), which is subsidized by The Netherlands Organization for the Advancement of Pure Research (ZWO). Initial experiments were supported by a grant to P.B. from NATO (grant Nr-559). A.C.C.F. gratefully acknowledges a research training grant from the World Health Organization (WHO No. M8/181/4/F.78).

PY - 1980/12/11

Y1 - 1980/12/11

N2 - We have compared a total of 30 recognition sites for eight restriction endonucleases on the 20-kilobase-pair maxi-circle of kinetoplast DNAs from five different Trypanosoma brucei strains. In addition to three polymorphic sites we have found a 5 kilobase-pair region that is not cleaved by any of the eight enzymes and that varies in size over 1 kilobase pair in the strains analysed. Mini-circles from these five strains, digested with endonuclease TaqI or MboII, yield very complex fragment patterns, showing that extensive mini-circle sequence heterogeneity is a common characteristic of these T. brucei strains. The size distribution of mini-circle fragments in these digests was identical for different clones of the 427 strain, but very different for mini-circles from different strains. These results show that maxi-circle sequence is conserved, whereas mini-circle sequence is not. Restriction digests of maxi-circles could be useful in determining how closely two Trypanosoma strains are related, whereas mini-circle digests can serve as sensitive tags for individual strains.

AB - We have compared a total of 30 recognition sites for eight restriction endonucleases on the 20-kilobase-pair maxi-circle of kinetoplast DNAs from five different Trypanosoma brucei strains. In addition to three polymorphic sites we have found a 5 kilobase-pair region that is not cleaved by any of the eight enzymes and that varies in size over 1 kilobase pair in the strains analysed. Mini-circles from these five strains, digested with endonuclease TaqI or MboII, yield very complex fragment patterns, showing that extensive mini-circle sequence heterogeneity is a common characteristic of these T. brucei strains. The size distribution of mini-circle fragments in these digests was identical for different clones of the 427 strain, but very different for mini-circles from different strains. These results show that maxi-circle sequence is conserved, whereas mini-circle sequence is not. Restriction digests of maxi-circles could be useful in determining how closely two Trypanosoma strains are related, whereas mini-circle digests can serve as sensitive tags for individual strains.

KW - (Trypanosoma brucei)

KW - Maxi-circle

KW - Mini-circle

KW - Nucleotide sequence

KW - Restriction endonuclease

KW - kinetoplast DNA

UR - https://www.scopus.com/pages/publications/0019126574

U2 - 10.1016/0005-2787(80)90001-5

DO - 10.1016/0005-2787(80)90001-5

M3 - Article

C2 - 6260147

AN - SCOPUS:0019126574

SN - 0005-2787

VL - 610

SP - 197

EP - 210

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 2

ER -

Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains

Abstract

Keywords

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