XFrom structure to systems: High-resolution, quantitative genetic analysis of RNA polymerase II

Hannes Braberg, Huiyan Jin, Erica A. Moehle, Yujia A. Chan, Shuyi Wang, Michael Shales, Joris J. Benschop, John H. Morris, Chenxi Qiu, Fuqu Hu, Leung K. Tang, James S. Fraser, Frank C.P. Holstege, Philip Hieter, Christine Guthrie, Craig D. Kaplan, Nevan J. Krogan

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)

Abstract

RNA polymerase II (RNAPII) lies at the core of dynamic control of gene expression. Using 53 RNAPII point mutants, we generated a point mutant epistatic miniarray profile (pE-MAP) comprising ∼60,000 quantitative genetic interactions in Saccharomyces cerevisiae. This analysis enabled functional assignment of RNAPII subdomains and uncovered connections between individual regions and other protein complexes. Using splicing microarrays and mutants that alter elongation rates in vitro, we found an inverse relationship between RNAPII speed and in vivo splicing efficiency. Furthermore, the pE-MAP classified fast and slow mutants that favor upstream and downstream start site selection, respectively. The striking coordination of polymerization rate with transcription initiation and splicing suggests that transcription rate is tuned to regulate multiple gene expression steps. The pE-MAP approach provides a powerful strategy to understand other multifunctional machines at amino acid resolution.

Original languageEnglish
Pages (from-to)X775-788
JournalCell
Volume154
Issue number4
DOIs
Publication statusPublished - 15 Aug 2013
Externally publishedYes

Fingerprint

Dive into the research topics of 'XFrom structure to systems: High-resolution, quantitative genetic analysis of RNA polymerase II'. Together they form a unique fingerprint.

Cite this