TY - JOUR
T1 - XTcf-3 transcription factor mediates β-catenin-induced axis formation in xenopus embryos
AU - Molenaar, Miranda
AU - Van De Wetering, Marc
AU - Oosterwegel, Mariette
AU - Peterson-Maduro, Josi
AU - Godsave, Susan
AU - Korinek, Vladimir
AU - Roose, Jeroen
AU - Destrée, Olivier
AU - Clevers, Hans
N1 - Funding Information:
We thank Dr. H. Bos for critically reading the manuscript, Drs. J. Huelsken and J. Behrens for β-catenin cDNAs, Dr. R. M. Harland for noggin cDNA, Dr. E. M. DeRobertis for goosecoid cDNA, Dr. D. A. Melton for the cDNA library, and Dr. P. Krieg for the pT7TS vector. H. C. is supported by a PIONIER and PROGRAM grant from NWO–GMW.
PY - 1996/8/9
Y1 - 1996/8/9
N2 - XTcf-3 is a maternally expressed Xenopus homolog of the mammalian HMG box factors Tcf-1 and Lef-1. The N-terminus of XTcf-3 binds to β-catenin. Microinjection of XTcf-3 mRNA in embryos results in nuclear translocation of β-catenin. The β-catenin-XTcf-3 complex activates transcription in a transient reporter gene assay, while XTcf-3 by itself is silent. N-terminal deletion of XTcf-3 (ΔN) abrogates the interaction with β-catenin, as well as the consequent transcription activation. This dominant-negative ΔN mutant suppresses the induction of axis duplication by microinjected β-catenin. It also suppresses endogenous axis specification upon injection into the dorsal blastomeres of a 4-cell-stage embryo. We propose that signaling by β-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes.
AB - XTcf-3 is a maternally expressed Xenopus homolog of the mammalian HMG box factors Tcf-1 and Lef-1. The N-terminus of XTcf-3 binds to β-catenin. Microinjection of XTcf-3 mRNA in embryos results in nuclear translocation of β-catenin. The β-catenin-XTcf-3 complex activates transcription in a transient reporter gene assay, while XTcf-3 by itself is silent. N-terminal deletion of XTcf-3 (ΔN) abrogates the interaction with β-catenin, as well as the consequent transcription activation. This dominant-negative ΔN mutant suppresses the induction of axis duplication by microinjected β-catenin. It also suppresses endogenous axis specification upon injection into the dorsal blastomeres of a 4-cell-stage embryo. We propose that signaling by β-catenin involves complex formation with XTcf-3, followed by nuclear translocation and activation of specific XTcf-3 target genes.
UR - https://www.scopus.com/pages/publications/0001003110
U2 - 10.1016/S0092-8674(00)80112-9
DO - 10.1016/S0092-8674(00)80112-9
M3 - Article
C2 - 8756721
AN - SCOPUS:0001003110
SN - 0092-8674
VL - 86
SP - 391
EP - 399
JO - Cell
JF - Cell
IS - 3
ER -