2-Adrenergic receptor agonists activate CFTR in intestinal organoids and subjects with cystic fibrosis

Lodewijk A.W. Vijftigschild, Gitte Berkers, Johanna F. Dekkers, Domenique D. Zomer-Van Ommen, Elizabeth Matthes, Evelien Kruisselbrink, Annelotte Vonk, Chantal E. Hensen, Sabine Heida-Michel, Margot Geerdink, Hettie M. Janssens, Eduard A. Van De Graaf, Inez Bronsveld, Karin M. De Winter-De Groot, Christof J. Majoor, Harry G.M. Heijerman, Hugo R. De Jonge, John W. Hanrahan, Cornelis K. Van Der Ent, Jeffrey M. Beekman

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

26 Citaten (Scopus)

Samenvatting

We hypothesized that people with cystic fibrosis (CF) who express CFTR (cystic fibrosis transmembrane conductance regulator) gene mutations associated with residual function may benefit from G-protein coupled receptor (GPCR)-targeting drugs that can activate and enhance CFTR function. We used intestinal organoids to screen a GPCR-modulating compound library and identified ?2-adrenergic receptor agonists as the most potent inducers of CFTR function. ?2-Agonist-induced organoid swelling correlated with the CFTR genotype, and could be induced in homozygous CFTR-F508del organoids and highly differentiated primary CF airway epithelial cells after rescue of CFTR trafficking by small molecules. The in vivo response to treatment with an oral or inhaled ?2-agonist (salbutamol) in CF patients with residual CFTR function was evaluated in a pilot study. 10 subjects with a R117H or A455E mutation were included and showed changes in the nasal potential difference measurement after treatment with oral salbutamol, including a significant improvement of the baseline potential difference of the nasal mucosa (+6.35 mV, p<0.05), suggesting that this treatment might be effective in vivo. Furthermore, plasma that was collected after oral salbutamol treatment induced CFTR activation when administered ex vivo to organoids. This proof-of-concept study suggests that organoids can be used to identify drugs that activate CFTR function in vivo and to select route of administration.

Originele taal-2Engels
Pagina's (van-tot)768-779
Aantal pagina's12
TijdschriftEuropean Respiratory Journal
Volume48
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 1 sep. 2016
Extern gepubliceerdJa

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