A Hierarchy of H3K4me3 and H3K27me3 Acquisition in Spatial Gene Regulation in Xenopus Embryos

Robert C. Akkers, Simon J. van Heeringen, Ulrike G. Jacobi, Eva M. Janssen-Megens, Kees Jan Françoijs, Hendrik G. Stunnenberg, Gert Jan C. Veenstra

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

184 Citaten (Scopus)

Samenvatting

Epigenetic mechanisms set apart the active and inactive regions in the genome of multicellular organisms to produce distinct cell fates during embryogenesis. Here, we report on the epigenetic and transcriptome genome-wide maps of gastrula-stage Xenopus tropicalis embryos using massive parallel sequencing of cDNA (RNA-seq) and DNA obtained by chromatin immunoprecipitation (ChIP-seq) of histone H3 K4 and K27 trimethylation and RNA Polymerase II (RNAPII). These maps identify promoters and transcribed regions. Strikingly, genomic regions featuring opposing histone modifications are mostly transcribed, reflecting spatially regulated expression rather than bivalency as determined by expression profile analyses, sequential ChIP, and ChIP-seq on dissected embryos. Spatial differences in H3K27me3 deposition are predictive of localized gene expression. Moreover, the appearance of H3K4me3 coincides with zygotic gene activation, whereas H3K27me3 is predominantly deposited upon subsequent spatial restriction or repression of transcriptional regulators. These results reveal a hierarchy in the spatial control of zygotic gene activation.

Originele taal-2Engels
Pagina's (van-tot)425-434
Aantal pagina's10
TijdschriftDevelopmental Cell
Volume17
Nummer van het tijdschrift3
DOI's
StatusGepubliceerd - 15 sep. 2009
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'A Hierarchy of H3K4me3 and H3K27me3 Acquisition in Spatial Gene Regulation in Xenopus Embryos'. Samen vormen ze een unieke vingerafdruk.

Citeer dit