A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

Bernadette Jeremiasse; Ravian L van Ineveld; Veerle Bok; Michiel Kleinnijenhuis; Sam de Blank; Maria Alieva; Hannah R Johnson; Esmée J van Vliet; Amber L Zeeman; Lianne M Wellens; Gerard Llibre-Palomar; Mario Barrera Román; Alessia Di Maggio; Johanna F Dekkers; Sabrina Oliveira; Alexander L Vahrmeijer; Jan J Molenaar; Marc Hwa Wijnen; Alida Fw van der Steeg; Ellen J Wehrens; Anne C Rios

doi:10.1038/s44321-024-00084-4

A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

Bernadette Jeremiasse, Ravian L van Ineveld, Veerle Bok, Michiel Kleinnijenhuis, Sam de Blank, Maria Alieva, Hannah R Johnson, Esmée J van Vliet, Amber L Zeeman, Lianne M Wellens, Gerard Llibre-Palomar, Mario Barrera Román, Alessia Di Maggio, Johanna F Dekkers, Sabrina Oliveira, Alexander L Vahrmeijer, Jan J Molenaar, Marc Hwa Wijnen, Alida Fw van der Steeg, Ellen J WehrensAnne C Rios

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Samenvatting

Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.

Originele taal-2	Engels
Pagina's (van-tot)	1495-1514
Aantal pagina's	20
Tijdschrift	EMBO molecular medicine
Volume	16
Nummer van het tijdschrift	7
DOI's	https://doi.org/10.1038/s44321-024-00084-4
Status	Gepubliceerd - jul. 2024
Extern gepubliceerd	Ja

Trefwoorden

Breast Cancer
Fluorescence-guided Surgery
Multi-spectral 3D Imaging
Neuroblastoma
Patient-derived Organoids

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10.1038/s44321-024-00084-4

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Jeremiasse, B., van Ineveld, R. L., Bok, V., Kleinnijenhuis, M., de Blank, S., Alieva, M., Johnson, H. R., van Vliet, E. J., Zeeman, A. L., Wellens, L. M., Llibre-Palomar, G., Barrera Román, M., Di Maggio, A., Dekkers, J. F., Oliveira, S., Vahrmeijer, A. L., Molenaar, J. J., Wijnen, M. H., van der Steeg, A. F., ... Rios, A. C. (2024). A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery. EMBO molecular medicine, 16(7), 1495-1514. https://doi.org/10.1038/s44321-024-00084-4

@article{6ec54f932c7b4ec08f6f290c837fe7ac,

title = "A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery",

abstract = "Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.",

keywords = "Breast Cancer, Fluorescence-guided Surgery, Multi-spectral 3D Imaging, Neuroblastoma, Patient-derived Organoids",

author = "Bernadette Jeremiasse and {van Ineveld}, {Ravian L} and Veerle Bok and Michiel Kleinnijenhuis and {de Blank}, Sam and Maria Alieva and Johnson, {Hannah R} and {van Vliet}, {Esm{\'e}e J} and Zeeman, {Amber L} and Wellens, {Lianne M} and Gerard Llibre-Palomar and {Barrera Rom{\'a}n}, Mario and {Di Maggio}, Alessia and Dekkers, {Johanna F} and Sabrina Oliveira and Vahrmeijer, {Alexander L} and Molenaar, {Jan J} and Wijnen, {Marc Hwa} and {van der Steeg}, {Alida Fw} and Wehrens, {Ellen J} and Rios, {Anne C}",

note = "{\textcopyright} 2024. The Author(s).",

year = "2024",

month = jul,

doi = "10.1038/s44321-024-00084-4",

language = "English",

volume = "16",

pages = "1495--1514",

journal = "EMBO molecular medicine",

issn = "1757-4676",

publisher = "Wiley-Blackwell",

number = "7",

}

Jeremiasse, B, van Ineveld, RL, Bok, V, Kleinnijenhuis, M, de Blank, S, Alieva, M, Johnson, HR, van Vliet, EJ, Zeeman, AL, Wellens, LM, Llibre-Palomar, G, Barrera Román, M, Di Maggio, A, Dekkers, JF, Oliveira, S, Vahrmeijer, AL, Molenaar, JJ, Wijnen, MH, van der Steeg, AF, Wehrens, EJ & Rios, AC 2024, 'A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery', EMBO molecular medicine, vol. 16, nr. 7, blz. 1495-1514. https://doi.org/10.1038/s44321-024-00084-4

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T1 - A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

AU - Jeremiasse, Bernadette

AU - van Ineveld, Ravian L

AU - Bok, Veerle

AU - Kleinnijenhuis, Michiel

AU - de Blank, Sam

AU - Alieva, Maria

AU - Johnson, Hannah R

AU - van Vliet, Esmée J

AU - Zeeman, Amber L

AU - Wellens, Lianne M

AU - Llibre-Palomar, Gerard

AU - Barrera Román, Mario

AU - Di Maggio, Alessia

AU - Dekkers, Johanna F

AU - Oliveira, Sabrina

AU - Vahrmeijer, Alexander L

AU - Molenaar, Jan J

AU - Wijnen, Marc Hwa

AU - van der Steeg, Alida Fw

AU - Wehrens, Ellen J

AU - Rios, Anne C

PY - 2024/7

Y1 - 2024/7

N2 - Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.

AB - Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.

KW - Breast Cancer

KW - Fluorescence-guided Surgery

KW - Multi-spectral 3D Imaging

KW - Neuroblastoma

KW - Patient-derived Organoids

UR - https://www.mendeley.com/catalogue/ecc08433-3a85-31f6-ae48-701639ffff48/

U2 - 10.1038/s44321-024-00084-4

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C2 - 38831131

SN - 1757-4676

VL - 16

SP - 1495

EP - 1514

JO - EMBO molecular medicine

JF - EMBO molecular medicine

IS - 7

ER -

A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery

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