TY - JOUR
T1 - A multispectral 3D live organoid imaging platform to screen probes for fluorescence guided surgery
AU - Jeremiasse, Bernadette
AU - van Ineveld, Ravian L.
AU - Bok, Veerle
AU - Kleinnijenhuis, Michiel
AU - de Blank, Sam
AU - Alieva, Maria
AU - Johnson, Hannah R.
AU - van Vliet, Esmée J.
AU - Zeeman, Amber L.
AU - Wellens, Lianne M.
AU - Llibre-Palomar, Gerard
AU - Barrera Román, Mario
AU - Di Maggio, Alessia
AU - Dekkers, Johanna F.
AU - Oliveira, Sabrina
AU - Vahrmeijer, Alexander L.
AU - Molenaar, Jan J.
AU - Wijnen, Marc H.W.A.
AU - van der Steeg, Alida F.W.
AU - Wehrens, Ellen J.
AU - Rios, Anne C.
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/7/15
Y1 - 2024/7/15
N2 - Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.
AB - Achieving complete tumor resection is challenging and can be improved by real-time fluorescence-guided surgery with molecular-targeted probes. However, pre-clinical identification and validation of probes presents a lengthy process that is traditionally performed in animal models and further hampered by inter- and intra-tumoral heterogeneity in target expression. To screen multiple probes at patient scale, we developed a multispectral real-time 3D imaging platform that implements organoid technology to effectively model patient tumor heterogeneity and, importantly, healthy human tissue binding.
KW - Breast Cancer
KW - Fluorescence-guided Surgery
KW - Multi-spectral 3D Imaging
KW - Neuroblastoma
KW - Patient-derived Organoids
UR - https://www.scopus.com/pages/publications/85195517233
U2 - 10.1038/s44321-024-00084-4
DO - 10.1038/s44321-024-00084-4
M3 - Article
C2 - 38831131
AN - SCOPUS:85195517233
SN - 1757-4676
VL - 16
SP - 1495
EP - 1514
JO - EMBO molecular medicine
JF - EMBO molecular medicine
IS - 7
ER -