Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations

Klaus Rehe, Kerrie Wilson, Simon Bomken, Daniel Williamson, Julie Irving, Monique L den Boer, Martin Stanulla, Martin Schrappe, Andrew G Hall, Olaf Heidenreich, Josef Vormoor

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

Leukaemia-propagating cells are more frequent in high-risk acute B lymphoblastic leukaemia than in many malignancies that follow a hierarchical cancer stem cell model. It is unclear whether this characteristic can be more universally applied to patients from non-'high-risk' sub-groups and across a broad range of cellular immunophenotypes. Here, we demonstrate in a wide range of primary patient samples and patient samples previously passaged through mice that leukaemia-propagating cells are found in all populations defined by high or low expression of the lymphoid differentiation markers CD10, CD20 or CD34. The frequency of leukaemia-propagating cells and their engraftment kinetics do not differ between these populations. Transcriptomic analysis of CD34(high) and CD34(low) blasts establishes their difference and their similarity to comparable normal progenitors at different stages of B-cell development. However, consistent with the functional similarity of these populations, expression signatures characteristic of leukaemia propagating cells in acute myeloid leukaemia fail to distinguish between the different populations. Together, these findings suggest that there is no stem cell hierarchy in acute B lymphoblastic leukaemia.

Originele taal-2Engels
Pagina's (van-tot)38-51
Aantal pagina's14
TijdschriftEMBO molecular medicine
Volume5
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - jan. 2013
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations'. Samen vormen ze een unieke vingerafdruk.

Citeer dit