Affinity purification of histidine-tagged proteins

Jacky Schmitt; Heike Hess; Hendrik G. Stunnenberg

doi:10.1007/BF01674434

Affinity purification of histidine-tagged proteins

Jacky Schmitt, Heike Hess, Hendrik G. Stunnenberg

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel › peer review

198 Citaten (Scopus)

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Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni²⁺-NTA chromatography.

Originele taal-2	Engels
Pagina's (van-tot)	223-230
Aantal pagina's	8
Tijdschrift	Molecular Biology Reports
Volume	18
Nummer van het tijdschrift	3
DOI's	https://doi.org/10.1007/BF01674434
Status	Gepubliceerd - okt. 1993
Extern gepubliceerd	Ja

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title = "Affinity purification of histidine-tagged proteins",

abstract = "Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA chromatography.",

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AB - Expression of recombinant proteins is a standard technique in molecular biology and a wide variety of prokaryotic as well as eukaryotic expression systems are currently in use. A limiting step is often the purification of the expressed recombinant protein, particularly if mammalian expression systems that yield low expression levels are employed. Here, we discuss the advantages and restrictions of tagging recombinant proteins with histidines and purifying them by Ni2+-NTA chromatography.

KW - affinity purification

KW - histidine

KW - nickel

KW - recombinant protein

KW - tagging

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Affinity purification of histidine-tagged proteins

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