An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers

Keyphrases

• Biallelic 100%
• Knock-in Mouse Model 100%
• Epigenetic Modifiers 100%
• Mouse Embryonic Stem Cells (mESCs) 100%
• Null mutant Mice 100%
• Embryonic Stem Cells 66%
• SET Domain Bifurcated 1 (SETDB1) 66%
• Genetic Background 33%
• Repurposing 33%
• High Efficiency 33%
• Biological Processes 33%
• Self-renewal 33%
• Epigenetic Regulators 33%
• Histone Demethylase 33%
• Growth Inhibition 33%
• Independent Pathway 33%
• Targeting Vector 33%
• Nanog 33%
• Human Embryonic Stem Cell Lines 33%
• Mutant Phenotype 33%
• Popular 33%
• Conditional Gene Deletion 33%
• Null Cell 33%
• Phenotype Reversion 33%
• Intermediate Vector 33%
• Retargeting 33%
• 2i Medium 33%
• Independent Network 33%
• Pluripotency Network 33%
• Naive Pluripotency 33%
• FLP Recombinase 33%
• Tamoxifen-inducible 33%

Biochemistry, Genetics and Molecular Biology

• Embryonic Stem Cell 100%
• Mouse Mutant 100%
• Allele 50%
• Knockout Mouse 50%
• Genetic Background 16%
• Biological Phenomena and Functions Concerning the Entire Organism 16%
• Growth Inhibition 16%
• Essential Gene 16%
• Revertant 16%
• Histone Methyltransferase 16%
• Independent Pathway 16%
• Stem Cell Line 16%
• Nanog 16%
• Recombinase 16%