An endometrial gene expression signature accurately predicts recurrent implantation failure after IVF

Yvonne E.M. Koot, Sander R. Van Hooff, Carolien M. Boomsma, Dik Van Leenen, Marian J.A.Groot Koerkamp, Mariëtte Goddijn, Marinus J.C. Eijkemans, Bart C.J.M. Fauser, Frank C.P. Holstege, Nick S. Macklon

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

144 Citaten (Scopus)

Samenvatting

The primary limiting factor for effective IVF treatment is successful embryo implantation. Recurrent implantation failure (RIF) is a condition whereby couples fail to achieve pregnancy despite consecutive embryo transfers. Here we describe the collection of gene expression profiles from mid-luteal phase endometrial biopsies (n = 115) from women experiencing RIF and healthy controls. Using a signature discovery set (n = 81) we identify a signature containing 303 genes predictive of RIF. Independent validation in 34 samples shows that the gene signature predicts RIF with 100% positive predictive value (PPV). The strength of the RIF associated expression signature also stratifies RIF patients into distinct groups with different subsequent implantation success rates. Exploration of the expression changes suggests that RIF is primarily associated with reduced cellular proliferation. The gene signature will be of value in counselling and guiding further treatment of women who fail to conceive upon IVF and suggests new avenues for developing intervention.

Originele taal-2Engels
Artikelnummer19411
TijdschriftScientific Reports
Volume6
DOI's
StatusGepubliceerd - 22 jan. 2016
Extern gepubliceerdJa

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