TY - JOUR
T1 - An HMG-box-containing T-cell factor required for thymocyte differentiation
AU - Verbeek, Sjef
AU - Izon, David
AU - Hofhuis, Frans
AU - Robanus-Maandag, Els
AU - Te Riele, Hein
AU - Watering, Marc Van De
AU - Oosterwegel, Mariette
AU - Wilson, Anne
AU - Robson MacDonald, H.
AU - Clevers, Hans
PY - 1995
Y1 - 1995
N2 - Two candidate genes for controlling thymocyte differentiation,T-cellfactor- 1 (Tcf-1) and lymphoid enhancer-binding factor (Lef-1), encode closely related DNA-binding HMG-box proteins1,2. Their expression pattern is complex and largely overlapping during embryogenesis, yet restricted to lymphocytes postnatally3. Here we generate two independent germline mutations in Tcf-1 and find that thymocyte development in (otherwise normal) mutant mice is blocked at the transition from the CD8+, immature single-positive to the CD4+/CD8+ double-positive stage. In contrast to wild-type mice, most of the immature single-positive cells in the mutants are not in the cell cycle and the number of immunocompetent T cells in peripheral lymphoid organs is reduced. We conclude that Tcf-1 controls an essential step in thymocyte differentiation.
AB - Two candidate genes for controlling thymocyte differentiation,T-cellfactor- 1 (Tcf-1) and lymphoid enhancer-binding factor (Lef-1), encode closely related DNA-binding HMG-box proteins1,2. Their expression pattern is complex and largely overlapping during embryogenesis, yet restricted to lymphocytes postnatally3. Here we generate two independent germline mutations in Tcf-1 and find that thymocyte development in (otherwise normal) mutant mice is blocked at the transition from the CD8+, immature single-positive to the CD4+/CD8+ double-positive stage. In contrast to wild-type mice, most of the immature single-positive cells in the mutants are not in the cell cycle and the number of immunocompetent T cells in peripheral lymphoid organs is reduced. We conclude that Tcf-1 controls an essential step in thymocyte differentiation.
UR - http://www.scopus.com/inward/record.url?scp=0028985161&partnerID=8YFLogxK
U2 - 10.1038/374070a0
DO - 10.1038/374070a0
M3 - Article
C2 - 7870176
AN - SCOPUS:0028985161
SN - 0028-0836
VL - 374
SP - 70
EP - 74
JO - Nature
JF - Nature
IS - 6517
ER -