TY - JOUR
T1 - An increase in CD62Ldim neutrophils precedes the development of pulmonary embolisms in COVID-19 patients
AU - the COVPACH study group
AU - Spijkerman, Roy
AU - Jorritsma, Nikita K.N.
AU - Bongers, Suzanne H.
AU - Bindels, Bas J.J.
AU - Jukema, Bernard N.
AU - Hesselink, Lillian
AU - Hietbrink, Falco
AU - Leenen, Luke P.H.
AU - van Goor, Harriët M.R.
AU - Vrisekoop, Nienke
AU - Kaasjager, Karin A.H.
AU - Koenderman, Leo
AU - Stiphout, Feike
AU - Nijdam, Thomas M.P.
AU - van de Ven, Nils L.M.
AU - Verhaegh, Remi
AU - Spanjaard, Judith S.
AU - Verboeket, Benjamin W.
AU - Laane, Duco
AU - van Wessem, Karlijn
AU - van spengler, Daan E.J.
AU - Buitenwerf, Wiebe
AU - Giustarini, Giulio
AU - Mulder, Eva
AU - Heijerman, Harry
AU - Zabaleta, Amely Daza
AU - van den bos, Frederique
AU - Rademaker, Emma
AU - Varkila, Meri R.J.
AU - de Mul, Nikki
AU - Cremer, Olaf L.
AU - Slooter, Arjen
AU - Delemarre, Eveline M.
AU - Nierkens, Stefan
AU - Limper, Maarten
AU - van Wijk, Femke
AU - Pandit, Aridaman
AU - Leavis, Helen
AU - Clark, Chantal C.
AU - Barendrecht, Arjan D.
AU - Seinen, Cor W.
AU - Drost-Verhoef, Sandra
AU - Smits, Simone
AU - Parr, Naomi M.J.
AU - Sebastian, Sylvie A.E.
AU - Koekman, Arnold C.
AU - van Wesel, Annet C.
AU - van der Vries, Erhard
AU - Maas, Coen
AU - de Maat, Steven
N1 - Publisher Copyright:
© 2021 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Scandinavian Foundation for Immunology.
PY - 2021/6
Y1 - 2021/6
N2 - Objectives: A high incidence of pulmonary embolism (PE) is reported in patients with critical coronavirus disease 2019 (COVID-19). Neutrophils may contribute to this through a process referred to as immunothrombosis. The aim of this study was to investigate the occurrence of neutrophil subpopulations in blood preceding the development of COVID-19 associated PE. Methods: We studied COVID-19 patients admitted to the ICU of our tertiary hospital between 19-03-2020 and 17-05-2020. Point-of-care fully automated flow cytometry was performed prior to ICU admission, measuring the neutrophil activation/maturation markers CD10, CD11b, CD16 and CD62L. Neutrophil receptor expression was compared between patients who did or did not develop PE (as diagnosed on CT angiography) during or after their ICU stay. Results: Among 25 eligible ICU patients, 22 subjects were included for analysis, of whom nine developed PE. The median (IQR) time between neutrophil phenotyping and PE occurrence was 9 (7-12) days. A significant increase in the immune-suppressive neutrophil phenotype CD16bright/CD62Ldim was observed on the day of ICU admission (P = 0.014) in patients developing PE compared to patients who did not. Conclusion: The increase in this neutrophil phenotype indicates that the increased number of CD16bright/CD62Ldim neutrophils might be used as prognostic marker to predict those patients that will develop PE in critical COVID-19 patients.
AB - Objectives: A high incidence of pulmonary embolism (PE) is reported in patients with critical coronavirus disease 2019 (COVID-19). Neutrophils may contribute to this through a process referred to as immunothrombosis. The aim of this study was to investigate the occurrence of neutrophil subpopulations in blood preceding the development of COVID-19 associated PE. Methods: We studied COVID-19 patients admitted to the ICU of our tertiary hospital between 19-03-2020 and 17-05-2020. Point-of-care fully automated flow cytometry was performed prior to ICU admission, measuring the neutrophil activation/maturation markers CD10, CD11b, CD16 and CD62L. Neutrophil receptor expression was compared between patients who did or did not develop PE (as diagnosed on CT angiography) during or after their ICU stay. Results: Among 25 eligible ICU patients, 22 subjects were included for analysis, of whom nine developed PE. The median (IQR) time between neutrophil phenotyping and PE occurrence was 9 (7-12) days. A significant increase in the immune-suppressive neutrophil phenotype CD16bright/CD62Ldim was observed on the day of ICU admission (P = 0.014) in patients developing PE compared to patients who did not. Conclusion: The increase in this neutrophil phenotype indicates that the increased number of CD16bright/CD62Ldim neutrophils might be used as prognostic marker to predict those patients that will develop PE in critical COVID-19 patients.
KW - CD62L
KW - COVID-19
KW - Intensive Care Unit
KW - L-selectin
KW - Neutrophils
KW - pulmonary embolism
KW - SARS-CoV-2
KW - Thrombosis
UR - http://www.scopus.com/inward/record.url?scp=85106264427&partnerID=8YFLogxK
U2 - 10.1111/sji.13023
DO - 10.1111/sji.13023
M3 - Article
C2 - 33482019
AN - SCOPUS:85106264427
SN - 0300-9475
VL - 93
JO - Scandinavian Journal of Immunology
JF - Scandinavian Journal of Immunology
IS - 6
M1 - e13023
ER -