An oestrogen-receptor-α-bound human chromatin interactome

Melissa J. Fullwood, Mei Hui Liu, You Fu Pan, Jun Liu, Han Xu, Yusoff Bin Mohamed, Yuriy L. Orlov, Stoyan Velkov, Andrea Ho, Poh Huay Mei, Elaine G.Y. Chew, Phillips Yao Hui Huang, Willem Jan Welboren, Yuyuan Han, Hong Sain Ooi, Pramila N. Ariyaratne, Vinsensius B. Vega, Yanquan Luo, Peck Yean Tan, Pei Ye ChoyK. D.Senali Abayratna Wansa, Bing Zhao, Kar Sian Lim, Shi Chi Leow, Jit Sin Yow, Roy Joseph, Haixia Li, Kartiki V. Desai, Jane S. Thomsen, Yew Kok Lee, R. Krishna Murthy Karuturi, Thoreau Herve, Guillaume Bourque, Hendrik G. Stunnenberg, Xiaoan Ruan, Valere Cacheux-Rataboul, Wing Kin Sung, Edison T. Liu, Chia Lin Wei, Edwin Cheung, Yijun Ruan

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1332 Citaten (Scopus)

Samenvatting

Genomes are organized into high-level three-dimensional structures, and DNA elements separated by long genomic distances can in principle interact functionally. Many transcription factors bind to regulatory DNA elements distant from gene promoters. Although distal binding sites have been shown to regulate transcription by long-range chromatin interactions at a few loci, chromatin interactions and their impact on transcription regulation have not been investigated in a genome-wide manner. Here we describe the development of a new strategy, chromatin interaction analysis by paired-end tag sequencing (ChIA-PET) for the de novo detection of global chromatin interactions, with which we have comprehensively mapped the chromatin interaction network bound by oestrogen receptor α (ER-α) in the human genome. We found that most high-confidence remote ER-α-binding sites are anchored at gene promoters through long-range chromatin interactions, suggesting that ER-α functions by extensive chromatin looping to bring genes together for coordinated transcriptional regulation. We propose that chromatin interactions constitute a primary mechanism for regulating transcription in mammalian genomes.

Originele taal-2Engels
Pagina's (van-tot)58-64
Aantal pagina's7
TijdschriftNature
Volume462
Nummer van het tijdschrift7269
DOI's
StatusGepubliceerd - 5 nov. 2009
Extern gepubliceerdJa

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