Analysis of mouse Rad54 expression and its implications for homologous recombination

Jeroen Essers, Rudi W. Hendriks, Joanna Wesoly, Cecile E.M.T. Beerens, Bep Smit, Jan H.J. Hoeijmakers, Claire Wyman, Mies L.G. Dronkert, Roland Kanaar

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

58 Citaten (Scopus)

Samenvatting

Homologous recombination is one of the major pathways for repair of DNA double-strand breaks (DSBs). Important proteins in this pathway are Rad51 and Rad54. Rad51 forms a nucleoprotein filament on single-stranded DNA (ssDNA) that mediates pairing with and strand invasion of homologous duplex DNA with the assist of Rad54. We estimated that the nucleus of a mouse embryonic stem (ES) cells contains on average 4.7×105 Rad51 and 2.4×105 Rad54 molecules. Furthermore, we showed that the amount of Rad54 was subject to cell cycle regulation. We discuss our results with respect to two models that describe how Rad54 stimulates Rad51-mediated DNA strand invasion. The models differ in whether Rad54 functions locally or globally. In the first model, Rad54 acts in cis relative to the site of strand invasion. Rad54 coats the Rad51 nucleoprotein filament in stoichiometric amounts and binds to the target duplex DNA at the site that is homologous to the ssDNA in the Rad51 nucleoprotein filament. Subsequently, it promotes duplex DNA unwinding. In the second model, Rad54 acts in trans relative to the site of strand invasion. Rad54 binds duplex DNA distant from the site that will be unwound. Translocation of Rad54 along the duplex DNA increases superhelical stress thereby promoting duplex DNA unwinding.

Originele taal-2Engels
Pagina's (van-tot)779-793
Aantal pagina's15
TijdschriftDNA Repair
Volume1
Nummer van het tijdschrift10
DOI's
StatusGepubliceerd - 1 okt. 2002
Extern gepubliceerdJa

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