Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF

Marc Van de Wetering, Robert Cavallo, Dennis Dooijes, Moniek Van Beest, Johan Van Es, Joseph Loureiro, Arne Ypma, Deborah Hursh, Tamara Jones, Amy Bejsovec, Mark Peifer, Mark Mortin, Hans Clevers

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

1069 Citaten (Scopus)

Samenvatting

The vertebrate transcription factors TCF (T cell factor) and LEF (lymphocyte enhancer binding factor) interact with β-catenin and are hypothesized to mediate Wingless/Wnt signaling. We have cloned a maternally expressed Drosophila TCF family member, dTCF- dTCF binds a canonical TCF DNA motif and interacts with the β-catenin homolog Armadillo. Previous studies have identified two regions in Armadillo required for Wingless signaling. One of these interacts with dTCF, while the other constitutes a transactivation domain. Mutations in dTCF and expression of a dominant-negative dTCF transgene cause a segment polarity phenotype and affect expression of the Wingless target genes engrailed and Ultrabithorax. Epistasis analysis positions dTCF downstream of armadillo. The Armadillo-dTCF complex mediates Wingless signaling as a bipartite transcription factor.

Originele taal-2Engels
Pagina's (van-tot)789-799
Aantal pagina's11
TijdschriftCell
Volume88
Nummer van het tijdschrift6
DOI's
StatusGepubliceerd - 21 mrt. 1997
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF'. Samen vormen ze een unieke vingerafdruk.

Citeer dit