TY - JOUR
T1 - Asparagine synthetase activity in paediatric acute leukaemias
T2 - AML-M5 subtype shows lowest activity
AU - Dübbers, A.
AU - Würthwein, G.
AU - Müller, H. J.
AU - Schulze-Westhoff, P.
AU - Winkelhorst, M.
AU - Kurzknabe, E.
AU - Lanvers, C.
AU - Pieters, R.
AU - Kaspers, G. J.L.
AU - Creutzig, U.
AU - Ritter, J.
AU - Boos, J.
PY - 2000
Y1 - 2000
N2 - Lack of sufficient cellular activity of asparagine synthetase (AS) in blast cells compared with normal tissue is thought to be the basis of the antileukaemic effect of L-asparaginase in acute lymphoblastic leukaemia (ALL). Although L-asparaginase is routinely used in ALL, its role and value in the treatment of acute myelogenous leukaemia (AML) is still being discussed. To evaluate the pharmacological basis for L-asparaginase treatment, we established pretreatment monitoring of the intracellular AS activity in blast cells of patients with AML and ALL. There was no general difference in AS activity between ALL and AML samples. Significantly lower AS activity, however, was found in the B-lineage ALL subgroups as well as AML- M5.
AB - Lack of sufficient cellular activity of asparagine synthetase (AS) in blast cells compared with normal tissue is thought to be the basis of the antileukaemic effect of L-asparaginase in acute lymphoblastic leukaemia (ALL). Although L-asparaginase is routinely used in ALL, its role and value in the treatment of acute myelogenous leukaemia (AML) is still being discussed. To evaluate the pharmacological basis for L-asparaginase treatment, we established pretreatment monitoring of the intracellular AS activity in blast cells of patients with AML and ALL. There was no general difference in AS activity between ALL and AML samples. Significantly lower AS activity, however, was found in the B-lineage ALL subgroups as well as AML- M5.
KW - Acute lymphoblastic leukaemia
KW - Acute myelogenous leukaemia
KW - Asparaginase
KW - Asparagine synthetase
KW - Childhood leukaemia
UR - http://www.scopus.com/inward/record.url?scp=0034039017&partnerID=8YFLogxK
U2 - 10.1046/j.1365-2141.2000.02015.x
DO - 10.1046/j.1365-2141.2000.02015.x
M3 - Article
C2 - 10848836
AN - SCOPUS:0034039017
SN - 0007-1048
VL - 109
SP - 427
EP - 429
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 2
ER -