TY - JOUR
T1 - Bioanalysis of HIV protease inhibitors in samples from sanctuary sites
AU - Crommentuyn, K. M.L.
AU - Huitema, A. D.R.
AU - Beijnen, J. H.
PY - 2005/6/1
Y1 - 2005/6/1
N2 - The human immunodeficiency virus (HIV) is present in several sites inside the human body, which are hardly accessible to antiretroviral drugs, the so-called sanctuary sites. The most important sanctuary sites are cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs) and seminal plasma. The determination of drug concentrations in these sanctuary sites may form an important step in treatment optimisation of HIV-infected individuals. However, bioanalysis in these sites is hampered by several factors with regard to sample preparation, chromatography and detection. In this review, we will discuss these issues and give an overview of published methods using high-performance liquid chromatography (HPLC) for the bioanalysis of HIV protease inhibitors in CSF, PBMCs and seminal plasma.
AB - The human immunodeficiency virus (HIV) is present in several sites inside the human body, which are hardly accessible to antiretroviral drugs, the so-called sanctuary sites. The most important sanctuary sites are cerebrospinal fluid (CSF), peripheral blood mononuclear cells (PBMCs) and seminal plasma. The determination of drug concentrations in these sanctuary sites may form an important step in treatment optimisation of HIV-infected individuals. However, bioanalysis in these sites is hampered by several factors with regard to sample preparation, chromatography and detection. In this review, we will discuss these issues and give an overview of published methods using high-performance liquid chromatography (HPLC) for the bioanalysis of HIV protease inhibitors in CSF, PBMCs and seminal plasma.
KW - Bioanalysis
KW - CSF
KW - HIV protease inhibitors
KW - HPLC
KW - PBMC
KW - Seminal plasma
UR - http://www.scopus.com/inward/record.url?scp=19444384722&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2004.12.012
DO - 10.1016/j.jpba.2004.12.012
M3 - Article
C2 - 15907632
AN - SCOPUS:19444384722
SN - 0731-7085
VL - 38
SP - 139
EP - 147
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 1
ER -