Bottom-up sample preparation for the LC-MS/MS quantification of anti-cancer monoclonal antibodies in bio matrices

Karen A.M. De Jong, Suse J. Van Breugel, Michel J.X. Hillebrand, Hilde Rosing, Alwin D.R. Huitema, Jos H. Beijnen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikel recenserenpeer review

8 Citaten (Scopus)

Samenvatting

Therapeutic monoclonal antibodies (mAbs) are rapidly taking over the treatment of many malignancies, and an astonishing number of mAbs is in development. This causes a high demand for quantification of mAbs in biomatrices both for measuring therapeutic mAb concentrations and to support pharmacokinetics and pharmacodynamics studies. Conventionally, ligand-binding assays are used for these purposes, but LC-MS is gaining popularity. Although intact (top-down) and subunit (middle-down) mAb quantification is reported, signature peptide (bottom-up) quantification is currently most advantageous. This review provides an overview of the reported bottom-up mAb quantification methods in biomatrices as well as general recommendations regarding signature peptide and internal standard selection, reagent use and optimization of digestion in bottom-up quantification methods.

Originele taal-2Engels
Pagina's (van-tot)1405-1425
Aantal pagina's21
TijdschriftBioanalysis
Volume12
Nummer van het tijdschrift19
DOI's
StatusGepubliceerd - okt. 2020
Extern gepubliceerdJa

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