TY - JOUR
T1 - Broad, but not universal, transcriptional requirement for yTAFII17, a histone H3-like TAFII present in TFIID and SAGA
AU - Apone, Lynne M.
AU - Virbasius, Ching Man A.
AU - Holstege, Frank C.P.
AU - Wang, Jing
AU - Young, Richard A.
AU - Green, Michael R.
N1 - Funding Information:
C. A. V. is supported by an NIH postdoctoral fellowship. M. R. G. is an investigator of the Howard Hughes Medical Institute. This work was supported in part by grants from the NIH to R. A. Y. and M. R. G. The genomic analysis experiments were supported in part by grants from the NIH, Bristol-Myers Squibb Company, Affymetrix Inc., and Millennium Pharmaceuticals, Inc.
PY - 1998/11
Y1 - 1998/11
N2 - The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA box-binding protein (TBP) and associated factors (TAFIIs). Experiments in yeast have shown that although most TAFIIs are required for viability, many genes are transcribed normally upon inactivation of individual and even multiple yTAFIIs. Here we analyze yTAFII17, recently found to be present in both the SAGA HAT complex as well as TFIID. Functional inactivation of yTAFII17 by temperature-sensitive mutation or depletion results in loss of transcription of many, but not all, genes. The upstream activating sequence (UAS), which contains the activator binding sites, is the region that renders a gene yTAFII17 dependent. In conjunction with previous studies, our results reveal that different TAFIIs have remarkably distinct properties.
AB - The RNA polymerase II general transcription factor TFIID is a multisubunit complex comprising TATA box-binding protein (TBP) and associated factors (TAFIIs). Experiments in yeast have shown that although most TAFIIs are required for viability, many genes are transcribed normally upon inactivation of individual and even multiple yTAFIIs. Here we analyze yTAFII17, recently found to be present in both the SAGA HAT complex as well as TFIID. Functional inactivation of yTAFII17 by temperature-sensitive mutation or depletion results in loss of transcription of many, but not all, genes. The upstream activating sequence (UAS), which contains the activator binding sites, is the region that renders a gene yTAFII17 dependent. In conjunction with previous studies, our results reveal that different TAFIIs have remarkably distinct properties.
UR - http://www.scopus.com/inward/record.url?scp=0032214124&partnerID=8YFLogxK
U2 - 10.1016/S1097-2765(00)80163-X
DO - 10.1016/S1097-2765(00)80163-X
M3 - Article
C2 - 9844637
AN - SCOPUS:0032214124
SN - 1097-2765
VL - 2
SP - 653
EP - 661
JO - Molecular Cell
JF - Molecular Cell
IS - 5
ER -