CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex

Cornelia G. Spruijt, Stefanie J.J. Bartels, Arie B. Brinkman, Jorrit V. Tjeertes, Ina Poser, Hendrik G. Stunnenberg, Michiel Vermeulen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

46 Citaten (Scopus)


The Mi-2/NuRD (NUcleosome Remodeling and histone Deacetylase) chromatin remodeling complex is a large heterogeneous multiprotein complex associated with transcriptional repression. Here we apply a SILAC based quantitative proteomics approach to show that all known Mi-2/NuRD complex subunits co-purify with Cyclin Dependent Kinase 2 Associated Protein1 (CDK2AP1), also known as Deleted in Oral Cancer 1 (DOC-1). DOC-1 displays in vitro binding affinity for methylated DNA as part of the meCpG binding MBD2/NuRD complex. In luciferase reporter assays, DOC-1 is a potent repressor of transcription. Finally, immunofluorescence experiments reveal co-localization between MBD2 and DOC-1 in mouse NIH-3T3 nuclei. Collectively, these results indicate that DOC-1 is a bona fide subunit of the Mi-2/NuRD chromatin remodeling complex.

Originele taal-2Engels
Pagina's (van-tot)1700-1706
Aantal pagina's7
TijdschriftMolecular BioSystems
Nummer van het tijdschrift9
StatusGepubliceerd - sep. 2010
Extern gepubliceerdJa


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