Cell sensitivity assays: The MTT assay

Johan Van Meerloo, Gertjan J.L. Kaspers, Jacqueline Cloos

Onderzoeksoutput: Hoofdstuk in Boek/Rapport/CongresprocedureHoofdstukpeer review

1662 Citaten (Scopus)

Samenvatting

The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity. Since for most cell populations the total mitochondrial activity is related to the number of viable cells, this assay is broadly used to measure the in vitro cytotoxic effects of drugs on cell lines or primary patient cells. In this chapter the protocol of the assay is described including important considerations relevant for each step of the assay as well as its limitations and possible applications.

Originele taal-2Engels
TitelCancer Cell Culture
SubtitelMethods and Protocols
UitgeverijHumana Press Inc.
Pagina's237-245
Aantal pagina's9
ISBN van geprinte versie9781617790799
DOI's
StatusGepubliceerd - 2011
Extern gepubliceerdJa

Publicatie series

NaamMethods in Molecular Biology
Volume731
ISSN van geprinte versie1064-3745

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