Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes

Nagesha A.S. Rao, Melysia T. McCalman, Panagiotis Moulos, Kees Jan Francoijs, Aristotelis Chatziioannou, Fragiskos N. Kolisis, Michael N. Alexis, Dimitra J. Mitsiou, Hendrik G. Stunnenberg

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

171 Citaten (Scopus)

Samenvatting

Glucocorticoid receptor (GR) exerts anti-inflammatory action in part by antagonizing proinflammatory transcription factors such as the nuclear factor kappa-b (NFKB). Here, we assess the crosstalk of activated GR and RELA (p65, major NFKB component) by global identification of their binding sites and target genes. We show that coactivation of GR and p65 alters the repertoire of regulated genes and results in their association with novel sites in a mutually dependent manner. These novel sites predominantly cluster with p65 target genes that are antagonized by activated GR and vice versa. Our data show that coactivation of GR and NFKB alters signaling pathways that are regulated by each factor separately and provide insight into the networks underlying the GR and NFKB crosstalk.

Originele taal-2Engels
Pagina's (van-tot)1404-1416
Aantal pagina's13
TijdschriftGenome Research
Volume21
Nummer van het tijdschrift9
DOI's
StatusGepubliceerd - sep. 2011
Extern gepubliceerdJa

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