Combined quantification of intracellular (phospho-)proteins and transcriptomics from fixed single cells

Jan P. Gerlach, Jessie A.G. van Buggenum, Sabine E.J. Tanis, Mark Hogeweg, Branco M.H. Heuts, Mauro J. Muraro, Lisa Elze, Francesca Rivello, Agata Rakszewska, Alexander van Oudenaarden, Wilhelm T.S. Huck, Hendrik G. Stunnenberg, Klaas W. Mulder

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

33 Citaten (Scopus)

Samenvatting

Environmental stimuli often lead to heterogeneous cellular responses and transcriptional output. We developed single-cell RNA and Immunodetection (RAID) to allow combined analysis of the transcriptome and intracellular (phospho-)proteins from fixed single cells. RAID successfully recapitulated differentiation-state changes at the protein and mRNA level in human keratinocytes. Furthermore, we show that differentiated keratinocytes that retain high phosphorylated FAK levels, a feature associated with stem cells, also express a selection of stem cell associated transcripts. Our data demonstrates that RAID allows investigation of heterogeneous cellular responses to environmental signals at the mRNA and phospho-proteome level.

Originele taal-2Engels
Artikelnummer1469
TijdschriftScientific Reports
Volume9
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 1 dec. 2019
Extern gepubliceerdJa

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