TY - JOUR
T1 - Comparison of cellular drug resistance in acute lymphoblastic leukemia in children and in adults
AU - Styczynski, J.
AU - Pieters, R.
AU - Huismans, D. R.
AU - Schuurhuis, G. J.
AU - Veerman, A. J.P.
AU - Wysocki, M.
PY - 1999
Y1 - 1999
N2 - The prognosis of acute lymphoblastic leukemia (ALL) in adults is poor in comparison to children with ALL. The aim of the study was to determine the drug resistance profile in lymphoblasts of adult ALL in comparison to paediatric ALL. Drug resistance profile was compared by means of the MTT assay in 10 adults and 11 children samples at initial presentation of ALL. The following 14 drugs were tested: cytosine arabinoside, L- asparaginase, dexamethasone, daunorubicin, doxorubicin, idarubicin, 4-HOO-ifosfamide, 6-mercaptopurine, mitoxantrone, prednisolone, teniposide, 6-thioguanine, vincristine and vepesid. Lymphoblasts from adult patients were more resistant to all tested drugs. For idarubicine, L-asparaginase and teniposide the results reached statistical difference. The resistance ratio of adult vs children ALL was the most prominent for L-asparaginase (43-fold), prednisolon (> 15.7-fold), teniposide (12-fold), vincristine (9,3-fold), daunorubicin (5,4-fold) and cytosine arabinoside (5,3-fold). The resistance profile expressed by combined resistance to prednisolon, vincristine and L- asparaginase (PVA score) may be a fundamental difference between adult and children ALL which may underlie its different biological aspects and explain the difference in prognosis.
AB - The prognosis of acute lymphoblastic leukemia (ALL) in adults is poor in comparison to children with ALL. The aim of the study was to determine the drug resistance profile in lymphoblasts of adult ALL in comparison to paediatric ALL. Drug resistance profile was compared by means of the MTT assay in 10 adults and 11 children samples at initial presentation of ALL. The following 14 drugs were tested: cytosine arabinoside, L- asparaginase, dexamethasone, daunorubicin, doxorubicin, idarubicin, 4-HOO-ifosfamide, 6-mercaptopurine, mitoxantrone, prednisolone, teniposide, 6-thioguanine, vincristine and vepesid. Lymphoblasts from adult patients were more resistant to all tested drugs. For idarubicine, L-asparaginase and teniposide the results reached statistical difference. The resistance ratio of adult vs children ALL was the most prominent for L-asparaginase (43-fold), prednisolon (> 15.7-fold), teniposide (12-fold), vincristine (9,3-fold), daunorubicin (5,4-fold) and cytosine arabinoside (5,3-fold). The resistance profile expressed by combined resistance to prednisolon, vincristine and L- asparaginase (PVA score) may be a fundamental difference between adult and children ALL which may underlie its different biological aspects and explain the difference in prognosis.
KW - Acute lymphoblastic leukemia
KW - Children, adults, PVA score
KW - Drug resistance
KW - MTT assay
UR - http://www.scopus.com/inward/record.url?scp=0032721446&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0032721446
SN - 0001-5814
VL - 30
SP - 283
EP - 290
JO - Acta Haematologica Polonica
JF - Acta Haematologica Polonica
IS - 3
ER -