@article{7a7f000b064142899cfcfb2f73f32b57,
title = "Controlled gene expression in primary Lgr5 organoid cultures",
abstract = "The study of gene function in endodermal epithelia such as of stomach, small intestine and colon relies heavily on transgenic approaches. Establishing such animal models is laborious, expensive and time-consuming. We present here a method based on Cre recombinaseinducible retrovirus vectors that allows the conditional manipulation of gene expression in primary mouse organoid culture systems.",
author = "Koo, {Bon Kyoung} and Stange, {Daniel E.} and Toshiro Sato and Wouter Karthaus and Farin, {Henner F.} and Meritxell Huch and {Van Es}, {Johan H.} and Hans Clevers",
note = "Funding Information: We thank L. Kaaij, P. Tetteh, H. Begthel and S. van den Brink for experimental help. Rosa-CreERT2 mice were donated by A. Smith (Wellcome Trust Centre for Stem Cell Research, University of Cambridge) and DN-MAML1 by J. Aster (Harvard Medical School). This work was funded in part by grants from the European Research Council, EU/232814-StemCeLLMark and the National Research Foundation of Korea, NRF-2011-357-C00093 (B.-K.K.), The Centre van Biomedical Genetic (D.E.S.), The Dutch Cancer Society, KWF/Hubr2007-3956 (T.S.), the EU FP7 Tornado-KBBE-222720 (W.K.), EMBO long-term fellowship (LTF to H.F.F.), the EU Marie Curie Fellowships, EU/236954-ICSC LGR5 (M.H.), Ti Pharma/T3-106 (J.H.E.) and the KNAW/3V-fund.",
year = "2012",
month = jan,
doi = "10.1038/nmeth.1802",
language = "English",
volume = "9",
pages = "81--83",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "1",
}