CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks; Hans Clevers; Benedetta Artegiani

doi:10.1016/j.stem.2020.10.014

CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

Delilah Hendriks, Hans Clevers, Benedetta Artegiani

Onderzoeksoutput: Bijdrage aan tijdschrift › Artikel recenseren › peer review

158 Citaten (Scopus)

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CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

Originele taal-2	Engels
Pagina's (van-tot)	705-731
Aantal pagina's	27
Tijdschrift	Cell stem cell
Volume	27
Nummer van het tijdschrift	5
DOI's	https://doi.org/10.1016/j.stem.2020.10.014
Status	Gepubliceerd - 5 nov. 2020

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10.1016/j.stem.2020.10.014

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abstract = "CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.",

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AU - Artegiani, Benedetta

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Y1 - 2020/11/5

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AB - CRISPR-Cas technology has revolutionized biological research and holds great therapeutic potential. Here, we review CRISPR-Cas systems and their latest developments with an emphasis on application to human cells. We also discuss how different CRISPR-based strategies can be used to accomplish a particular genome engineering goal. We then review how different CRISPR tools have been used in genome engineering of human stem cells in vitro, covering both the pluripotent (iPSC/ESC) and somatic adult stem cell fields and, in particular, 3D organoid cultures. Finally, we discuss the progress and challenges associated with CRISPR-based genome editing of human stem cells for therapeutic use.

KW - CRISPR-Cas Systems/genetics

KW - Clustered Regularly Interspaced Short Palindromic Repeats/genetics

KW - Gene Editing

KW - Genetic Engineering

KW - Humans

KW - Organoids

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JO - Cell stem cell

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CRISPR-Cas Tools and Their Application in Genetic Engineering of Human Stem Cells and Organoids

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