Diabetes risk gene and wnt effector Tcf7l2/TCF4 controls hepatic response to perinatal and adult metabolic demand

Sylvia F. Boj, Johan H. Van Es, Meritxell Huch, Vivian S.W. Li, Anabel José, Pantelis Hatzis, Michal Mokry, Andrea Haegebarth, Maaike Van Den Born, Pierre Chambon, Peter Voshol, Yuval Dor, Edwin Cuppen, Cristina Fillat, Hans Clevers

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

181 Citaten (Scopus)

Samenvatting

Most studies on TCF7L2 SNP variants in the pathogenesis of type 2 diabetes (T2D) focus on a role of the encoded transcription factor TCF4 in β cells. Here, a mouse genetics approach shows that removal of TCF4 from β cells does not affect their function, whereas manipulating TCF4 levels in the liver has major effects on metabolism. In Tcf7l2-/- mice, the immediate postnatal surge in liver metabolism does not occur. Consequently, pups die due to hypoglycemia. By combining chromatin immunoprecipitation with gene expression profiling, we identify a TCF4-controlled metabolic gene program that is acutely activated in the postnatal liver. In concordance, adult liver-specific Tcf7l2 knockout mice show reduced hepatic glucose production during fasting and display improved glucose homeostasis when maintained on high-fat diet. Furthermore, liver-specific TCF4 overexpression increases hepatic glucose production. These observations imply that TCF4 directly activates metabolic genes and that inhibition of Wnt signaling may be beneficial in metabolic disease.

Originele taal-2Engels
Pagina's (van-tot)1595-1607
Aantal pagina's13
TijdschriftCell
Volume151
Nummer van het tijdschrift7
DOI's
StatusGepubliceerd - 21 dec. 2012
Extern gepubliceerdJa

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