TY - JOUR
T1 - Diagnostic value of OCT3/4 for pre-invasive and invasive testicular germ cell tumours
AU - de Jong, Jeroen
AU - Stoop, Hans
AU - Dohle, Gert R
AU - Bangma, Chris H
AU - Kliffen, Mike
AU - van Esser, Joost W J
AU - van den Bent, Martin
AU - Kros, Johan M
AU - Oosterhuis, J Wolter
AU - Looijenga, Leendert H J
N1 - Copyright 2005 Pathological Society of Great Britain and Ireland
PY - 2005/6
Y1 - 2005/6
N2 - Human testicular germ cell tumours of adolescents and adults (TGCTs), the seminomatous and non-seminomatous germ cell tumours, show morphological and biological similarities to normal embryonic development, presumably determined by their supposed cell of origin, the primordial germ cell/gonocyte. Based on this knowledge, OCT3/4, also known as POU5F1, was recently defined as a diagnostic marker for these tumour types. In the adult testis, positive immunohistochemistry for OCT3/4 is an absolute indicator for the presence of the TGCT precursor carcinoma in situ/intratubular germ cell neoplasia undifferentiated (CIS/ITGCNU), seminoma, and/or embryonal carcinoma. Several studies have confirmed this observation, using the same polyclonal antibody. The present study demonstrates the usefulness of OCT3/4 immunohistochemistry in a diagnostic setting of a consecutively collected series of more than 200 testicular tumours and over 80 testicular biopsies. Moreover, it is shown that a monoclonal antibody directed against OCT3/4 is as informative as the polyclonal antibody, both in immunohistochemistry and in western blot analysis. The antibodies are robust and applicable with different methods of pretreatment and storage of tissue. This allows routine application of this diagnostic marker.
AB - Human testicular germ cell tumours of adolescents and adults (TGCTs), the seminomatous and non-seminomatous germ cell tumours, show morphological and biological similarities to normal embryonic development, presumably determined by their supposed cell of origin, the primordial germ cell/gonocyte. Based on this knowledge, OCT3/4, also known as POU5F1, was recently defined as a diagnostic marker for these tumour types. In the adult testis, positive immunohistochemistry for OCT3/4 is an absolute indicator for the presence of the TGCT precursor carcinoma in situ/intratubular germ cell neoplasia undifferentiated (CIS/ITGCNU), seminoma, and/or embryonal carcinoma. Several studies have confirmed this observation, using the same polyclonal antibody. The present study demonstrates the usefulness of OCT3/4 immunohistochemistry in a diagnostic setting of a consecutively collected series of more than 200 testicular tumours and over 80 testicular biopsies. Moreover, it is shown that a monoclonal antibody directed against OCT3/4 is as informative as the polyclonal antibody, both in immunohistochemistry and in western blot analysis. The antibodies are robust and applicable with different methods of pretreatment and storage of tissue. This allows routine application of this diagnostic marker.
KW - Adult
KW - Antibodies, Monoclonal/immunology
KW - Biomarkers, Tumor/analysis
KW - Blotting, Western
KW - DNA-Binding Proteins/analysis
KW - Female
KW - Humans
KW - Immunoenzyme Techniques
KW - Male
KW - Neoplasm Invasiveness
KW - Neoplasm Proteins/analysis
KW - Neoplasms, Germ Cell and Embryonal/diagnosis
KW - Octamer Transcription Factor-3
KW - Seminoma/diagnosis
KW - Testicular Neoplasms/diagnosis
KW - Transcription Factors/analysis
UR - http://www.scopus.com/inward/record.url?scp=19944391945&partnerID=8YFLogxK
U2 - 10.1002/path.1766
DO - 10.1002/path.1766
M3 - Article
C2 - 15818593
SN - 0022-3417
VL - 206
SP - 242
EP - 249
JO - The Journal of pathology
JF - The Journal of pathology
IS - 2
ER -