TY - JOUR
T1 - Differences between rodent and human cell lines in the amount of integrated DNA after transfection
AU - Hoeijmakers, Jan H.J.
AU - Odijk, Hanny
AU - Westerveld, Andries
N1 - Funding Information:
This study was supported financially by EURATGM (contract no. BI6-141-NL) and FUNGG, Foundation of Medical Scientific Research in The Netherlands (contract 900-501-091).
PY - 1987/3
Y1 - 1987/3
N2 - The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.
AB - The suitability of Chinese hamster and human cell lines for DNA-mediated gene transformation was investigated with respect to two parameters: the average quantity of and the integrity of integrated exogenous DNA fragments. No large differences were observed between most cell lines concerning the extent of fragmentation of the transferred DNA molecules. By contrast, the average number of sequences stably incorporated by the human cells (four lines tested) was 20- to 100-fold lower than the average amount inserted in the five Chinese hamster lines investigated. The very low uptake exhibited by the human cells, ranging from less than 100 up to 500 kb, renders these cells less suitable for transfection with genomic DNA to isolate specific genes.
UR - http://www.scopus.com/inward/record.url?scp=0023136672&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(87)90230-8
DO - 10.1016/0014-4827(87)90230-8
M3 - Article
C2 - 3028842
AN - SCOPUS:0023136672
SN - 0014-4827
VL - 169
SP - 111
EP - 119
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -