Disease variants alter transcription factor levels and methylation of their binding sites

the BIOS Consortium

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291 Citaten (Scopus)

Samenvatting

Most disease-associated genetic variants are noncoding, making it challenging to design experiments to understand their functional consequences. Identification of expression quantitative trait loci (eQTLs) has been a powerful approach to infer the downstream effects of disease-associated variants, but most of these variants remain unexplained. The analysis of DNA methylation, a key component of the epigenome, offers highly complementary data on the regulatory potential of genomic regions. Here we show that disease-associated variants have widespread effects on DNA methylation in trans that likely reflect differential occupancy of trans binding sites by cis-regulated transcription factors. Using multiple omics data sets from 3,841 Dutch individuals, we identified 1,907 established trait-associated SNPs that affect the methylation levels of 10,141 different CpG sites in trans (false discovery rate (FDR) < 0.05). These included SNPs that affect both the expression of a nearby transcription factor (such as NFKB1, CTCF and NKX2-3) and methylation of its respective binding site across the genome. Trans methylation QTLs effectively expose the downstream effects of disease-associated variants.

Originele taal-2Engels
Pagina's (van-tot)131-138
Aantal pagina's8
TijdschriftNature Genetics
Volume49
Nummer van het tijdschrift1
DOI's
StatusGepubliceerd - 1 jan. 2017
Extern gepubliceerdJa

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