Disruption of TTDA Results in Complete Nucleotide Excision Repair Deficiency and Embryonic Lethality

Arjan F. Theil, Julie Nonnekens, Barbara Steurer, Pierre Olivier Mari, Jan de Wit, Charlène Lemaitre, Jurgen A. Marteijn, Anja Raams, Alex Maas, Marcel Vermeij, Jeroen Essers, Jan H.J. Hoeijmakers, Giuseppina Giglia-Mari, Wim Vermeulen

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

32 Citaten (Scopus)

Samenvatting

The ten-subunit transcription factor IIH (TFIIH) plays a crucial role in transcription and nucleotide excision repair (NER). Inactivating mutations in the smallest 8-kDa TFB5/TTDA subunit cause the neurodevelopmental progeroid repair syndrome trichothiodystrophy A (TTD-A). Previous studies have shown that TTDA is the only TFIIH subunit that appears not to be essential for NER, transcription, or viability. We studied the consequences of TTDA inactivation by generating a Ttda knock-out (Ttda-/-) mouse-model resembling TTD-A patients. Unexpectedly, Ttda-/- mice were embryonic lethal. However, in contrast to full disruption of all other TFIIH subunits, viability of Ttda-/- cells was not affected. Surprisingly, Ttda-/- cells were completely NER deficient, contrary to the incomplete NER deficiency of TTD-A patient-derived cells. We further showed that TTD-A patient mutations only partially inactivate TTDA function, explaining the relatively mild repair phenotype of TTD-A cells. Moreover, Ttda-/- cells were also highly sensitive to oxidizing agents. These findings reveal an essential role of TTDA for life, nucleotide excision repair, and oxidative DNA damage repair and identify Ttda-/- cells as a unique class of TFIIH mutants.

Originele taal-2Engels
Artikelnummere1003431
TijdschriftPLoS Genetics
Volume9
Nummer van het tijdschrift4
DOI's
StatusGepubliceerd - apr. 2013
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Disruption of TTDA Results in Complete Nucleotide Excision Repair Deficiency and Embryonic Lethality'. Samen vormen ze een unieke vingerafdruk.

Citeer dit