TY - JOUR
T1 - Distinct differences in binding capacity to saccharide epitopes in supratentorial pilocytic astrocytomas, astrocytomas, anaplastic astrocytomas, and glioblastornas
AU - Camby, Isabelle
AU - Decaestecker, Christine
AU - Gordower, Laurence
AU - Dedecker, Robert
AU - Kacem, Yasmine
AU - Lemmers, Arnaud
AU - Siebert, Hans Christian
AU - Bovin, Nicolai V.
AU - Wesseling, Pieter
AU - Danguy, Andr
AU - Salmon, Isabelle
AU - Gabius, Hans Joachim
AU - Kiss, Robert
PY - 2001
Y1 - 2001
N2 - We monitored the expression of glycan-binding sites on a panel of 10 biotinylated neoglycoconjugates by means of quantitative computer-assisted microscopy to further study the molecular mechanisms in the extensive infiltration of the surrounding brain parenchyma by most astrocytic tumors. Three distinct histological compartments were analyzed for each of the 108 astrocytic tumors (15 pilocytic astrocytomas (WHO grade I), 25 astrocytomas (WHO grade II), 30 anaplastic astrocytomas (WHO grade III), and 38 glioblastomas (WHO grade IV) included in our series. These compartments were tumors (nonperivascular tumor astrocytes), perivascular tumor astrocytes, and blood vessel walls. Clear differences were observed between the pilocytic and the diffuse astrocytic tumors. Furthermore, malignant progression in the latter category was paralleled by a decrease in cells' ability to bind distinct sugar epitopes, especially the D-GalNAc(αl-3)-D-CalNAc-β1-R determinant of the Forssman pentasaccharide in tumors, the α-L-fucose in perivascular tumor areas, and the β-D-glucose in tumor vessel walls. Markedly, the level of binding site expression for α-D-mannose decreased in the tumors, the perivascular tumor areas, and the vessel walls. These glycohistochemical results imply the functional relevance of protein-carbohydrate interactions in this tumor system.
AB - We monitored the expression of glycan-binding sites on a panel of 10 biotinylated neoglycoconjugates by means of quantitative computer-assisted microscopy to further study the molecular mechanisms in the extensive infiltration of the surrounding brain parenchyma by most astrocytic tumors. Three distinct histological compartments were analyzed for each of the 108 astrocytic tumors (15 pilocytic astrocytomas (WHO grade I), 25 astrocytomas (WHO grade II), 30 anaplastic astrocytomas (WHO grade III), and 38 glioblastomas (WHO grade IV) included in our series. These compartments were tumors (nonperivascular tumor astrocytes), perivascular tumor astrocytes, and blood vessel walls. Clear differences were observed between the pilocytic and the diffuse astrocytic tumors. Furthermore, malignant progression in the latter category was paralleled by a decrease in cells' ability to bind distinct sugar epitopes, especially the D-GalNAc(αl-3)-D-CalNAc-β1-R determinant of the Forssman pentasaccharide in tumors, the α-L-fucose in perivascular tumor areas, and the β-D-glucose in tumor vessel walls. Markedly, the level of binding site expression for α-D-mannose decreased in the tumors, the perivascular tumor areas, and the vessel walls. These glycohistochemical results imply the functional relevance of protein-carbohydrate interactions in this tumor system.
KW - Astrocytoma
KW - Glioblastoma
KW - Glycohistochemistry
KW - Neoangiogenesis
KW - Neoglycoconjugat e
KW - Sugar code
UR - http://www.scopus.com/inward/record.url?scp=0035140133&partnerID=8YFLogxK
U2 - 10.1093/jnen/60.1.75
DO - 10.1093/jnen/60.1.75
M3 - Article
C2 - 11202177
AN - SCOPUS:0035140133
SN - 0022-3069
VL - 60
SP - 75
EP - 84
JO - Journal of Neuropathology and Experimental Neurology
JF - Journal of Neuropathology and Experimental Neurology
IS - 1
ER -