TY - JOUR
T1 - DNA damage stabilizes interaction of CSB with the transcription elongation machinery
AU - Van Den Boom, Vincent
AU - Citterio, Elisabetta
AU - Hoogstraten, Deborah
AU - Zotter, Angelika
AU - Egly, Jean Marc
AU - Van Cappellen, Wiggert A.
AU - Hoeijmakers, Jan H.J.
AU - Houtsmuller, Adriaan B.
AU - Vermeulen, Wim
PY - 2004/7/5
Y1 - 2004/7/5
N2 - The Cockayne syndrome B (CSB) protein is essential for transcription- coupled DNA repair (TCR), which is dependent on RNA polymerase II elongation. TCR is required to quickly remove the cytotoxic transcription-blocking DNA lesions. Functional GFP-tagged CSB, expressed at physiological levels, was homogeneously dispersed throughout the nucleoplasm in addition to bright nuclear foci and nucleolar accumulation. Photobleaching studies showed that GFP-CSB, as part of a high molecular weight complex, transiently interacts with the transcription machinery. Upon (DNA damage-induced) transcription arrest CSB binding these interactions are prolonged, most likely reflecting actual engagement of CSB in TCR. These findings are consistent with a model in which CSB monitors progression of transcription by regularly probing elongation complexes and becomes more tightly associated to these complexes when TCR is active.
AB - The Cockayne syndrome B (CSB) protein is essential for transcription- coupled DNA repair (TCR), which is dependent on RNA polymerase II elongation. TCR is required to quickly remove the cytotoxic transcription-blocking DNA lesions. Functional GFP-tagged CSB, expressed at physiological levels, was homogeneously dispersed throughout the nucleoplasm in addition to bright nuclear foci and nucleolar accumulation. Photobleaching studies showed that GFP-CSB, as part of a high molecular weight complex, transiently interacts with the transcription machinery. Upon (DNA damage-induced) transcription arrest CSB binding these interactions are prolonged, most likely reflecting actual engagement of CSB in TCR. These findings are consistent with a model in which CSB monitors progression of transcription by regularly probing elongation complexes and becomes more tightly associated to these complexes when TCR is active.
KW - Cockayne syndrome
KW - GFP
KW - Photobleaching studies
KW - TCR
UR - http://www.scopus.com/inward/record.url?scp=3142739449&partnerID=8YFLogxK
U2 - 10.1083/jcb.200401056
DO - 10.1083/jcb.200401056
M3 - Article
C2 - 15226310
AN - SCOPUS:3142739449
SN - 0021-9525
VL - 166
SP - 27
EP - 36
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 1
ER -