DNA damage triggers nucleotide excision repair-dependent monoubiquitylation of histone H2A

Steven Bergink, Florian A. Salomons, Deborah Hoogstraten, Tom A.M. Groothuis, Harm De Waard, Junxin Wu, Li Yuan, Elisabetta Citterio, Adriaan B. Houtsmuller, Jacques Neefjes, Jan H.J. Hoeijmakers, Wim Vermeulen, Nico P. Dantuma

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

212 Citaten (Scopus)


Chromatin changes within the context of DNA repair remain largely obscure. Here we show that DNA damage induces monoubiquitylation of histone H2A in the vicinity of DNA lesions. Ultraviolet (UV)-induced monoubiquitylation of H2A is dependent on functional nucleotide excision repair and occurs after incision of the damaged strand. The ubiquitin ligase Ring2 is required for the DNA damage-induced H2A ubiquitylation. UV-induced ubiquitylation of H2A is dependent on the DNA damage signaling kinase ATR (ATM- and Rad3-related) but not the related kinase ATM (ataxia telangiectasia-mutated). Although the response coincides with phosphorylation of variant histone H2AX, H2AX was not required for H2A ubiquitylation. Together our data show that monoubiquitylation of H2A forms part of the cellular response to UV damage and suggest a role of this modification in DNA repair-induced chromatin remodeling.

Originele taal-2Engels
Pagina's (van-tot)1343-1352
Aantal pagina's10
TijdschriftGenes and Development
Nummer van het tijdschrift10
StatusGepubliceerd - 15 mei 2006
Extern gepubliceerdJa


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