Drosophila Tcf and Groucho interact to repress wingless signalling activity

Robert A. Cavallo, Rachel T. Cox, Melissa M. Moline, Jeroen Roose, Gordon A. Polevoy, Hans Clevers, Mark Peifer, Amy Bejsovec

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

608 Citaten (Scopus)

Samenvatting

Wingless/Wnt signalling directs cell-fate choices during embryonic development. Inappropriate reactivation of the pathway causes cancer. In Drosophila, signal transduction from Wingless stabilizes cytosolic Armadillo, which then forms a bipartite transcription factor with the HMG-box protein Drosophila Tcf (dTcf) and activates expression of Wingless-responsive genes. Here we report that in the absence of Armadillo, dTcf acts as a transcriptional repressor of Wingless-responsive genes, and we show that Groucho acts as a corepressor in this process. Reduction of dTcf activity partially suppresses wingless and armadillo mutant phenotypes, leading to derepression of Wingless-responsive genes. Furthermore, overexpression of wild-type dTcf enhances the phenotype of a weak wingless allele. Finally, mutations in the Drosophila groucho gene also suppress wingless and armadillo mutant phenotypes as Groucho physically interacts with dTcf and is required for its full repressor activity.

Originele taal-2Engels
Pagina's (van-tot)604-608
Aantal pagina's5
TijdschriftNature
Volume395
Nummer van het tijdschrift6702
DOI's
StatusGepubliceerd - 8 okt. 1998
Extern gepubliceerdJa

Vingerafdruk

Duik in de onderzoeksthema's van 'Drosophila Tcf and Groucho interact to repress wingless signalling activity'. Samen vormen ze een unieke vingerafdruk.

Citeer dit