Endothelial progenitor cells in diabetic patients with myocardial infarction - can statins improve their function?

Natália António, Ana Soares, Rosa Fernandes, Francisco Soares, Ana Lopes, Tiago Carvalheiro, Artur Paiva, Luís A Providência, Lino Gonçalves, Carlos Fontes Ribeiro

Onderzoeksoutput: Bijdrage aan tijdschriftArtikelpeer review

Samenvatting

The effect of statins on endothelial progenitor cells (EPCs) function derived from diabetic patients (DMpts) with acute myocardial infarction (AMI) is unknown. In this study we assess the response of early and late EPCs from diabetic versus non-diabetic patients (NDMpts) with AMI to statins. EPCs were obtained from 10 diabetic and 10 age-matched non-diabetic male patients with AMI. For each patient, cultures of early and late EPCs were performed under 4 different conditions: normal glucose concentration (control); high glucose concentration; normal glucose concentration with atorvastatin supplementation and normal glucose concentration with pravastatin supplementation. To compare the effect of these treatments on EPC function in DMpts versus NDMpts, we performed in vitro: EPC colony-forming units (CFU) assay; cell cycle analysis; viability assessment and expression of the surface markers CXCR4, CD133, CD34 and KDR. Under control conditions, CFU numbers were reduced in DMpts-derived EPCs when compared to those of NDMpts (1.4±0.8 vs 2.6±1.2 CFU/well, P=0.021). When early EPCs from DMpts were cultured in the presence of statins, CFU capacity was restored, surmounting that of NDMpts under control conditions. Statins significantly improved viability of early EPCs and delayed the onset of late EPCs senescence, even in cells from DMpts. In addition, statins induced approximately a 2-fold increase in the proportion of late EPCs in S-phase of the cell cycle (P<0.05). Statins have a beneficial effect on both early and late EPCs from DMpts with AMI. Despite the functional impairment of EPCs from DMpts, they exhibit similar responsiveness to statins as equivalent cells from NDMpts.

Originele taal-2Engels
Pagina's (van-tot)25-36
Aantal pagina's12
TijdschriftEuropean journal of pharmacology
Volume741
DOI's
StatusGepubliceerd - 15 okt. 2014
Extern gepubliceerdJa

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